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儿童咽拭子标本406份中EB病毒的检测及其基因型鉴定
引用本文:张小团,肖勇健,刘卓然,刘佳倪.儿童咽拭子标本406份中EB病毒的检测及其基因型鉴定[J].中国医药,2014(6):890-893.
作者姓名:张小团  肖勇健  刘卓然  刘佳倪
作者单位:南华大学附属第二医院检验科,湖南省衡阳市421001
基金项目:湖南省科学技术厅科技计划(2012FJ3103)
摘    要:目的了解湖南省株洲、湘潭和衡阳地区儿童鼻咽部EB病毒的感染状况及其基因型,为EB病毒的治疗、预防和临床检验提供指导。方法从株洲、湘潭和衡阳部分医院收集疑似EB病毒感染的儿童咽拭子标本406份,采用荧光定量聚合酶联反应(FQ—PCR)检测标本中的EB病毒-DNA;阳性标本分别用特异性引物从中扩增3个目的基因片段,其中PCR扩增的EBNA-3C片段直接电泳以分析1/2分型,BamHI片段和BamHI WI/I1交界区基因片段采用PCR-RFLP分析鉴定F/f和C/D分型,测序和Blast比对验汪分型结果。结果从406份标本中共检出 EB病毒-DNA阳性株159份,株洲74份(38.5%),其中1型72份(97.3%),2型2份(2.7%);湘潭42份(38.9%),1型40份(95.2%),2型2份(4.8%);衡阳43份(40.6%),1型43份(100%),2型未发现、从159份阳性株中随机扩增了73份做F/f分型,68份做C/D分型,共检测到F型73份,C型68份,3个地区均未检测到f型和D型。结论株洲,湘潭和衡阳儿童鼻咽部EB病毒感染的检出率分别为38.5%,38.9%,40.6%,且其主要感染的3种独立基因型以1、C、F型为主,3个地区间儿童鼻咽部EB病毒-DNA的检出率及其基因型的差异无统计学意义。

关 键 词:EB病毒  基因分型  儿童  咽拭子

Detection and genotyping identification of Epstein-Barr virus nasopharyngeal swabs in children
Zhang Xiaotuan. Xiao Yongjian,Liu Zhuoran,Liu Jiani.Detection and genotyping identification of Epstein-Barr virus nasopharyngeal swabs in children[J].China Medicine,2014(6):890-893.
Authors:Zhang Xiaotuan Xiao Yongjian  Liu Zhuoran  Liu Jiani
Institution:.( Department of Clinical Laboratory, Second Affiliated Hospital, University of South China, Hunan Province, Hengyang 421001, China)
Abstract:Objective To know the state and genotyping of Epstein-Barr virus(EBV) in~ection of naso- pharyngeal swabs in children. Methods Totally 406 samples were detected for EBV-DNA by RQ-PCR. The samples were collected fi'om nasopharyngeal swabs of children passibly suffering from Epstein-Ban" Virus infection in Zhuzhou, Xiangtan and Hengyang cities in Hunan province in China. The analysis of genotypes was imp|emented on three regions of EBV genome; EBNA-3C, BamHl WI/II and BamHI Fwere detected by the polymerase chain reaction (PCR) and restriction fragment length polymorphism ( RFLP). Results Totally 159 clinical strains were i- dentified fi'om children nasopharyngeal swabs. The EBV positive strains were detected in 74 of 192, 42 of 108,43 of 106 samples from Zhuzhou, Xiangtan and Hengyang, respectively. The genotype I was proven in 74/192, 42/ 108, 43/106 clinical samples in those areas, respectively. The frequencies of genotype F and genotype C were de- tected in all cases ( 100% ). Conclusions The frequencies of EBV-DNA are 38.5% , 38.9% and 40.6% ; genotypes l , C and F are the predonfinant genotyping of EBV. The frequencies of EBV-DNA and the type 1/2, type C/D, type F/f are not significantly different.
Keywords:Epstein-Barr virus  Genotype  Child  Nasopharynx
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