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| 苦参碱对人增生性瘢痕成纤维细胞增殖的影响 | |
| 引用本文： | 汤苏阳,蔡宝仁,黄高升,艾玉峰,张琳西. 苦参碱对人增生性瘢痕成纤维细胞增殖的影响[J]. 中国美容医学, 2001, 10(1): 6-8,F003 |
| 作者姓名： | 汤苏阳 蔡宝仁 黄高升 艾玉峰 张琳西 |
| 作者单位： | 1. 沈阳军区205医院烧伤整形科 2. 第四军医大学病理教研室 3. 第四军医大学西京医院整形外科中心 |
| 摘 要： | 目的：研究苦参碱(Matrine，简称Mat)对人增生性瘢痕成纤维细胞增殖的影响。方法：将Mat以不同浓度梯度作用于体外培养的增生性瘢痕成纤维细胞，测MTT反应的A^0值及LDH值，用免疫组织化学检测Mat用药前后的细胞核内增殖性抗原(proliferatmg cell nuclear antigen，PCNA)的表达。结果：增生性瘢痕成纤维细胞在Mat0．25～100mg／ml范围内呈剂量依赖性增殖抑制，但LDH值无明显改变；PCNA的表达，在Mat作用后明显减弱。结论：Mat在体外能明显抑制增生性瘢痕成纤维细胞的增殖而不引起细胞坏死性改变。 |
| 关 键 词： | 苦参碱 人增生性瘢痕 成纤维细胞 细胞增殖 细胞核内增殖性抗原 免疫组织化学 检测 |
| 文章编号： | 1008-6455 (2001) 01-0006-03 |
THE INFLUENCE OF MATRINE ON THE GROWTH AND PROLIFERATION OF SCAR-DERIVED FIBROBLASTS |
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| TANG Su yang CAI Bao ren HUANG Gao sheng. THE INFLUENCE OF MATRINE ON THE GROWTH AND PROLIFERATION OF SCAR-DERIVED FIBROBLASTS[J]. Chinese Journal of Aesthetic Medicine, 2001, 10(1): 6-8,F003 | |
| Authors: | TANG Su yang CAI Bao ren HUANG Gao sheng |
| Affiliation: | Jin Zhou 121001 |
| Abstract: | Objective: To study the influence of Matrine on growth and proliferation of fibroblasts derived hgpertropnic scar. Methods: The MTT OD value and the con tent of LDH was examined by biochemical analysis after Matrine was added to the monolayer culture system of scar-derived fibroblasts. The expressions of prolifrating cell nuclear antigen (PCNA)were examinedby immunochemied methed. Results:Mat at 0.10～100.mg/ml could decrease the MTT OD value in dose dependent manner amd could not increase the content of LDH significantly.The expression of PCNA in Mat groupswas highr than the control groups. Conclusion:Mat could inhibit the proliferation of derived hypertropnicscar fibroblasts rarely produce the toxicity on scar-derived fibroblasts. |
| Keywords: | Matrine Fibroblast Proliferation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |