牙周炎组织中miRNA表达谱差异筛选及功能预测分析

目的 筛选牙周炎患者组织中的差异表达miRNA,探讨其生物学功能以及参与的信号通路。方法 通过对微阵列数据库GSE54710中的158例牙周炎患者和40例健康人的牙龈组织中的基因芯片数据进行生物信息学分析,筛选差异表达miRNA,并预测参与的生物学功能和信号通路。采用SPSS 19.0软件包对数据进行统计学分析。结果 5种miRNAs（hsa-miR-451、hsa-miR-223、hsa-miR-486-5p、hsa-miR-3917、hsa-miR-671-5p）显著上调,4种miRNAs（hsa-miR-203、hsa-miR-210、hsa-miR-1246、hsa-miR-1260 ）显著下调。其中,hsa-miR-1260的靶基因584个,hsa-miR-451的靶基因139个。KEGG通路富集分析显示,hsa -miR-1260靶基因显著富集到TGF-beta等12条信号通路,hsa-miR-451靶基因显著富集到17条信号通路。结论 得到牙周炎组织中miRNAs的表达谱,牙周炎诱导的hsa-miR-1260和hsa-miR-451可能在牙周炎的生理病理学中起到关键作用。

Differential screening and functional prediction analysis of miRNA expression profiles in periodontitis

PURPOSE: To detect the gene expression of miRNAs in patients with periodontitis and to explore their biological functions and involved signaling pathways. METHODS: Bioinformatics analysis of gene chip data from 158 periodontitis patients and 40 healthy controls of the microarray database GSE54710 were performed. The expression changes of miRNAs were analyzed. The involved biological function and signal path was predicted. SPSS 19.0 software package was used for statistical analysis. RESULTS: Five miRNAs (hsa-miR-451, hsa-miR-223, hsa-miR-486-5p, hsa-miR-3917, hsa-miR-671-5p) were significantly up-regulated, and 4 miRNAs (hsa-miR-203, hsa-miR-210, hsa-miR-1246, hsa-miR-1260) were significantly down-regulated. Among them, there were 584 target genes of hsa-miR-1260 and 139 target genes of hsa-miR-451. KEGG pathway enrichment analysis showed that hsa-miR-1260 target gene was significantly enriched into 12 signaling pathways such as TGF-beta, and hsa-miR-451 target gene was significantly enriched into 17 signaling pathways. CONCLUSIONS: miRNAs expression profiles were obtained in periodontitis tissues, periodontitis-induced hsa-miR-1260 and hsa-miR-451 may play a key role in the pathophysiology of periodontitis.