CD44v6与COX-2在腮腺多形性腺瘤及癌在多形性腺瘤中的表达及临床意义

目的:探讨CD44v6与环氧合酶2(cyclooxygenase-2,COX-2)的表达与腮腺多形性腺瘤复发和恶化的相关性。方法:采用免疫组织化学法检测19例正常腮腺组织、25例初发多形性腺瘤、15例第一次复发多形性腺瘤、15例第二次复发多形性腺瘤和13例癌在多形性腺瘤组织中CD44v6与COX-2的表达,采用SPSS 15.0软件包分析CD44v6与COX-2与腮腺多形性腺瘤复发和恶化的相关性。结果:正常腮腺组织、初发多形性腺瘤、第一次复发多形性腺瘤、第二次复发多形性腺瘤与癌在多形性腺瘤中CD44v6阳性表达率分别为5.26%、44.00%、60.00%、93.37%和100.00%,COX-2阳性表达率分别为10.53%、40.00%、63.33%、93.37%和100.00%,各组CD44v6和COX-2阳性表达强度比较,差异有统计学意义(P<0.05)。正常腮腺组织中CD44v6和COX-2阳性表达率和表达强度显著低于其他各组(P<0.05),初发多形性腺瘤与第一次复发多形性腺瘤组织中CD44v6阳性表达率和表达强度比较均无统计学差异(P>0.05),2组COX-2表达强度比较无统计学差异(P>0.05),但COX-2阳性表达率差异显著(P<0.05);第二次复发多形性腺瘤组织中,CD44v6阳性表达率和表达强度以及COX-2表达强度显著高于第一次复发多形性腺瘤(P<0.05),但2组COX表达阳性率无显著差异(P>0.05);第二次复发多形性腺瘤组织与癌在多形性腺瘤组织中,CD44v6和COX-2阳性表达率和表达强度无统计学差异(P>0.05)。结论:CD44v6和COX-2能够通过协同作用,共同促进多形性腺瘤发生、侵袭、复发和恶化。

Expression of CD44v6 and COX-2 in pleomorphic adenomas and carcinomas of parotid gland

PURPOSE: To investigate the correlation between CD44v6 and cyclooxygenase-2(COX-2) expression and recurrence and deterioration of pleomorphic adenoma of parotid gland. METHODS: Immunohistochemistry was used to detect the expression of CD44v6 and COX-2 in 19 normal parotid tissues, 25 primary pleomorphic adenomas, 15 first recurrent pleomorphic adenoma, 15 second recurrent pleomorphic adenoma and 13 cancer in pleomorphic adenomas. The correlation between CD44v6 and COX-2 and the recurrence and deterioration of pleomorphic adenomas was evaluated using SPSS 15.0 software package. RESULTS: The positive expression rate of CD44v6 in normal parotid gland, primary pleomorphic adenoma, first recurrent pleomorphic adenoma, second recurrent pleomorphic adenoma and cancer was 5.26%, 44.00%, 60.00%, 93.37% and 100.00%, respectively;and the positive expression rate of COX-2 was 10.53%, 40.00%, 63.33%, 93.37% and 100.00%, respectively. There was significant difference in the positive expression intensity of CD44v6 and COX-2 among the groups (P<0.05). The positive expression rate and intensity of CD44v6 and COX-2 in normal parotid gland tissue were significantly lower than those in other groups (P<0.05). There was no significant difference in the positive expression rate and intensity of CD44v6 between primary pleomorphic adenoma and first recurrent pleomorphic adenoma (P>0.05). There was no significant difference in the expression intensity of COX-2 between the two groups (P>0.05), but the positive expression rate of COX-2 between the two groups was not significant(P>0.05). The positive expression rate and intensity of CD44v6 and COX-2 in the second recurrent pleomorphic adenoma were significantly higher than those in the first recurrent pleomorphic adenoma(P<0.05), but there was no significant difference between the two groups(P>0.05). CD44v6 in the second recurrent pleomorphic adenoma and cancer was significantly higher than that in the first recurrent pleomorphic adenoma(P<0.05). There was no significant difference in the expression rate and intensity of COX-2 (P>0.05). CONCLUSIONS: CD44v6 and COX-2 may play an important role in promoting occurrence, invasion, recurrence and deterioration of pleomorphic adenoma through synergistic action.