sST2/IL-33与Th17/Treg相关细胞因子在原发免疫性血小板减少症中的作用研究

目的 观察可溶性ST2（sST2）和白细胞介素（IL）-33在原发免疫性血小板减少症（ITP）中的表达水平变化及其对Th17/Treg细胞平衡的调节机制。方法 30例ITP患者作为ITP组，选取同期健康体检者20例作为对照组，采用酶联免疫吸附试验（ELISA）检测 2组外周血血清中 IL-33、sST-2、IL-17和转化生长因子-β（TGF-β）的水平。结果 与对照组比较，ITP组血清sST2及IL-17水平均升高（P＜0.01），TGF-β表达水平降低（P＜0.01），而IL-33的表达水平差异无统计学意义（P＞0.05）；在 ITP 组中 sST2 与 TGF-β 呈负相关（r=-0.471,P＜0.01），与 IL-17 无相关性（r=0.189，P＞0.05）。结论 sST2表达水平增加和Th17/Treg细胞失衡可能是导致ITP发病的免疫机制之一。

Study on the sST2/IL-33 and Th17/Treg related cytokine in patients with Primary Immune Thrombocytopenia

Objective To observe the changes of expression levels of serum soluble ST2 (sST2), interleukin-33 (IL-33), and their regulation effects on Th17 / Treg cell immune balance in primary immune thrombocytopenia (ITP), and to investigate the possible immune mechanism of sST2 and IL-33 in ITP. Methods A total of 30 patients with ITP were regarded as ITP group, and 20 healthy subjects were used as healthy control group. ELISA method was used to detect the serum levels of sST2, IL-33, IL-17 and TGF-β in two groups. Results Compared with the normal control group, the serum levels of sST2 and IL-17 were increased (P＜0.01), the serum level of TGF-β was decreased in ITP group (P＜0.01). There was no significant change in serum level of IL-33 between the two groups (P＞0.05). The correlation analysis showed that sST2 was negatively correlated with TGF-β in ITP group (r=-0.471, P＜0.01), and there was no correlation in sST2 and IL-17 (r=0.189, P＞0.05). Conclusion The increased expression of sST2 and the imbalance of Th17/Treg may be one of the key immunomechanisms of ITP.