过表达miR-218对舌鳞癌细胞系SCC-4和SCC-9的增殖和侵袭的影响

目的:探讨miR-218在舌鳞癌细胞中的表达,以及对细胞增殖、凋亡和侵袭的影响。方法:在人舌鳞癌细胞系SCC-4和SCC-9中分别转染negative control、miR-218模拟物和抑制剂,然后利用MTT法检测细胞增殖活性;通过流式细胞仪检测细胞周期和细胞凋亡;通过Transwell实验检测细胞侵袭能力,采用GraphPad 7.0软件包对数据进行统计学分析。结果:与对照组相比,转染miR-218抑制剂的SCC-4和SCC-9细胞,细胞增殖、细胞周期、细胞凋亡和侵袭能力无显著改变;而转染miR-218模拟物的细胞,增殖能力变弱,凋亡数增加,侵袭能力显著降低。结论:miR-218在口腔癌中的表达量和细胞增殖、细胞周期、细胞凋亡和侵袭能力相关,提示miR-218与口腔癌的发生、发展有一定关系。

Effects of microRNA-218 on proliferation,apoptosis and invasion of human tongue cancer cell line SCC-4 and SCC-9

PURPOSE: To study the effects of miR-218 on cell proliferation, apoptosis and invasion of human tongue cancer cell line SCC-4 and SCC-9 cells. METHODS: SCC-4 and SCC-9 cells were transfected with negative control, miR-218 mimics and inhibitor, then cell proliferation was detected by MTT assay, cell cycle and cell apoptosis were measured by flow cytometry, cell invasion ability was tested by Transwell assay. The data were analyzed using GraphPad 7.0 software package. RESULTS: Compared with control cells, there was no significant difference for cell proliferation, cell cycle, cell apoptosis and invasion ability in cells transfected with miR-218 inhibitor. However, decreased cell proliferation and invasion ability and increased apoptosis ratio were found in cells transfected with miR-218 mimics. CONCLUSIONS: The expression level of miR-218 in tongue cancer cell lines may be correlated with cell proliferation, apoptosis and invasion.