双硫仑对胰腺癌细胞增殖、迁移及侵袭的影响

目的:研究双硫仑对胰腺癌细胞MIAPaCa-2、PANC-1增殖、迁移及侵袭能力的影响。方法:体外培养胰腺癌细胞MIAPaCa-2和PANC-1，以CCK-8法检测不同浓度（0、2、4、8、12、16、20、24、32 μg/ml）双硫仑处理24 h对胰腺癌细胞增殖能力的影响。以双硫仑处理组为实验组，无药物组作为对照组，通过划痕实验、Transwell迁移及侵袭实验检测双硫仑对胰腺癌细胞迁移和侵袭能力的影响。结果:CCK-8实验结果显示:不同浓度（2、4、8、12、16、20、24、32 μg/ml）双硫仑处理组的胰腺癌细胞活力均低于 0 μg/ml双硫仑的对照组；细胞划痕实验结果显示:双硫仑处理组MIAPaCa-2、PANC-1细胞划痕面积改变率（S%）明显低于对应的对照组，差异均具有统计学意义(P<0.05)；Transwell 迁移实验结果显示:双硫仑处理组MIAPaCa-2、PANC-1穿膜的细胞数明显少于对应的对照组，差异均具有统计学意义(P<0.05)；Transwell 侵袭实验结果显示:双硫仑处理组MIAPaCa-2、PANC-1穿膜细胞数也明显少于对应的对照组，差异均具有统计学意义(P<0.05)。结论:双硫仑可以明显抑制胰腺癌细胞MIAPaCa-2和PANC-1的增殖、迁移及侵袭能力。

Effect of disulfiram on proliferation，migration and invasion of human pancreatic cancer cell

Objective:To investigate the effect of disulfiram on the proliferation，migration and invasion of pancreatic cancer cell line MIAPaCa-2 and PANC-1.Methods:Pancreatic cancer cell line MIAPaCa-2 and PANC-1 were cultured in vitro，and the effects of different concentration（0，2，4，8，12，16，20，24，32 μg/ml）of disulfiram were detected by Cell Counting Kit-8（CCK-8) assay.Wound healing assay，Transwell migration and invasion assay were employed to determine the migration and invasion ability of pancreatic cancer cells.Results:CCK-8 assay showed the viability of different concentration（2，4，8，12，16，20，24，32 μg/ml）of disulfiram treated cells were lower than the control.The results of wound healing assay showed that the change rate of wound area of disulfiram treated cells were smaller than the control（P<0.05).According to the results of Transwell migration assay，migration cell number of disulfiram treated cells were less than the control（P<0.05).The results of Transwell invasion assay showed that invasion cell number of disulfiram treated cells were also less than the control（P<0.05).Conclusion:Disulfiram could inhibit the proliferation，migration and invasion ability of pancreatic cancer cell line MIAPaCa-2 and PANC-1.