特异性沉默EPB49对结直肠癌细胞转移能力的影响以及作用机制

The effect of specific silencing EPB49 on cell metastasis of colorectal cancer and its mechanism

Objective:To investigate the effect of small interfering RNA(siRNA) silencing erthrocyte brane protein band 4.9（EPB49）on proliferation，invasion and migration of colorectal cancer SW480 cells and its possible mechanism.Methods:Colorectal cancer SW480 cells were divided into blank control group，NC control group(transfected negative control siRNA-NC)，siRNA-EPB49 group.siRNA-NC and siRNA-EPB49 were transfected into SW480 cells by Lipofectamine 2000.The proliferation activity of the cells was detected by CCK-8 experiment.Transwell assay was used to detect the invasion and migration of SW480 cells.Western Blot was used to detect the expression STAT3(p-STAT3)，just another kinase 2(JAK2)，and phosphated JAK2(p-JAK2) protein.Results:After silencing EPB49 expression，the expression of EPB49 protein in siRNA-EPB49 group was significantly decreased than that in blank control group(P<0.05)，and the cell proliferation activity，invasion and migration ability increased significantly(P<0.05).The results of Western Blot showed that the expression of p-STAT3 and p-JAK2 in SW480 cells increased significantly after EPB49 silenced(P<0.05).Conclusion:Silencing EPB49 can promote the proliferation，invasion and migration of colorectal cancer cells，which is related to the JAK2/STAT3 signaling pathway.