Effect of near-infrared light on in vitro cellular ATP production of osteoblasts and fibroblasts and on fracture healing with intramedullary fixation |
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| Affiliation: | 1. Research Scientist, Neurology, Medical College of Wisconsin, USA;2. Child Neurology Fellow, Medical College of Wisconsin, USA;3. Research Technologist, Neurology, Medical College of Wisconsin, USA;4. Assistant Professor, Research Office, Medical College of Wisconsin, USA;5. Assistant Professor, Radiology, Medical College of Wisconsin, USA;6. Bleser Professor of Neurology, Medical College of Wisconsin, USA;1. Bosch Institute, University of Sydney, NSW, 2006, Australia;2. Discipline of Physiology, University of Sydney, NSW, 2006, Australia;3. Melbourne Medical School, University of Melbourne, Parkville, VIC, 3010, Australia;4. Department of Anatomy and Neuroscience, University of Melbourne, Parkville, VIC, 3010, Australia;5. Discipline of Anatomy & Histology, University of Sydney, NSW, 2006, Australia;6. Neuroscience Research Australia and University of New South Wales, Randwick, NSW, 2031, Australia;7. Brain and Mind Centre, University of Sydney, Camperdown, NSW, 2050, Australia;1. Department of Psychiatry, University of Maryland School of Medicine, Baltimore, MD 21201-1549, United States;2. Psychiatry Residency Training Program, St. Elizabeths Hospital, Washington, DC 20032, United States;3. Division of Child & Adolescent Psychiatry, Department of Psychiatry, University of Maryland School of Medicine, Baltimore, MD 21201-1549, United States;4. Division of Endocrinology, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201-1549, United States;5. Department of Ophthalmology and Visual Science, University of Maryland School of Medicine, Baltimore, MD 21201-1549, United States;6. Department of Epidemiology and Public Health, University of Maryland School of Medicine, Baltimore, MD 21201-1549, United States;7. Department of Psychological Science, University of Vermont, Burlington, VT 05405-0134, United States;8. Center for Preventive Cardiology, University of Maryland School of Medicine, Baltimore, MD 21201-1549, United States;9. Department of Neurology, St. Elizabeths Hospital, Washington, DC 20032, United States;10. Rocky Mountain MIRECC, Denver, CO 80220, United States;11. VISN 5 MIRECC, Baltimore, MD 21201 United States;1. Chief Technology Officer, LiteCure LLC, New Castle, DE, United States;2. Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, United States;3. Department of Dermatology, Harvard Medical School, Boston, MA, United States |
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| Abstract: | ObjectiveEvaluate the effect of near-infrared light (NIR) on immediate production of ATP by osteoblasts and fibroblasts in vitro, and the healing process of rat femur fractures with intramedullary fixation.BackgroundNIR is one potential treatment option for complications of fracture healing, which has shown to stimulate cellular proliferation and to enhance the healing process.MethodsCell culture – MC3T3-E1 and 3T3-A31 cells were subjected to NIR at 660 nm, 830 nm, or both combined. ATP was assayed at 5, 10, 20, and 45 min after exposure. Animal study – 18 rats had surgery with retrograde intramedullary pins inserted into their femurs, which then underwent closed, transverse femur fracture. Rats were randomly divided into 3 study groups of 6 each: nonirradiated controls, 660 nm, and 830 nm NIR. Healing process was assessed by a blinded radiologist, assigning a healing score of 1–6 for radiographs taken on days 0, 7, 14, and 21.ResultsCell culture – All groups gave significant increase in ATP within 5–10 min, with decay to baseline by 45 min. 660 nm NIR was significantly more effective than 830 nm with fibroblasts or either wavelength with osteoblasts. Animal study – A significant increase in the fracture healing grade in the 660 nm group at day 14, but with no differences at day 21.ConclusionThe study demonstrated an immediate increase in ATP production in vitro and an initial acceleration of callus formation in the fracture healing process, in the presence of NIR. |
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| Keywords: | Near-infrared light (NIR) Fracture healing Cellular ATP production Radiographic scoring Clinical application |
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