ANXA1对口腔鳞癌TPF化疗敏感性的影响及作用机制探讨

目的:探讨ANXA1在口腔鳞癌TPF化疗中的作用机制。方法:构建ANXA1过表达及低表达细胞系,通过细胞增殖、细胞毒性实验、实时荧光定量PCR和Western免疫印迹方法,分析ANXA1在TPF化疗中的作用,并探讨ANXA1通过上皮-间质转化(EMT)通路在TPF化疗中的作用机制。采用SPSS18.0软件包对数据进行统计学分析。结果:过表达口腔鳞癌细胞系ANXA1后,细胞生长速率降低,细胞周期减慢,对TPF诱导化药物敏感性降低,口腔鳞癌细胞出现EMT;低表达口腔鳞癌细胞系ANXA1后,细胞生长速率增快,细胞周期加快,对TPF化疗药物敏感性增加,口腔鳞癌细胞出现逆EMT。结论:在口腔鳞癌TPF化疗中,ANXA1过表达导致细胞出现EMT,对化疗敏感性降低。

The effect and mechanism of ANXA1 on TPF chemosensitivity in oral squamous cell carcinoma

PURPOSE: To investigate the mechanism of ANXA1 in TPF chemotherapy of oral squamous cell carcinoma (OSCC). Methods: ANXA1 overexpression and low-expression cell lines were constructed. The role of ANXA1 in TPF chemotherapy was analyzed by cell proliferation, cytotoxicity test, real-time PCR and Western blot, and the mechanism of ANXA1 in TPF chemotherapy through EMT (epithelial-mesenchymal transition) pathway was discussed. The data were analyzed with SPSS 18.0 software package. Results: After overexpression of ANXA1, cell growth rate decreased, cell cycle slowed down, sensitivity to TPF-induced drugs decreased, and EMT occurred in OSCC. After underexpression of ANXA1, cell growth rate increased, cell cycle accelerated, sensitivity to TPF chemotherapeutic drugs increased, and reverse EMT occurred in OSCC. Conclusions: In TPF chemotherapy of OSCC, overexpression of ANXA1 results in EMT of cells, which leads to decreased chemosensitivity.