Construction and phenotypic characterization of M68, an RruI quorum sensing knockout mutant of the photosynthetic alphaproteobacterium Rhodospirillum rubrum |
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| Institution: | 1. CNRS, LCPME, UMR7564, 405 rue de Vandoeuvre, F-54600 Villers-lès-Nancy, France;2. Université de Lorraine, LCPME, UMR7564, 405 rue de Vandoeuvre, F-54600 Villers-lès-Nancy, France;3. Microbiology, Saarland University, Campus, Geb. A1.5, D-66123 Saarbruecken, Germany;1. Department of Hepatobiliary Surgery, Wenzhou Central Hospital, Wenzhou 325000, China;2. Environmental and Public Health School of Wenzhou Medical University, Wenzhou 325000, China;3. Department of Anus & Intestine Surgery, Wenzhou Central Hospital, Wenzhou, Zhejiang 325000, China;4. Department of Hepatobiliary Surgery, The First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, China |
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| Abstract: | Many bacterial species communicate using a complex system known as quorum sensing (QS) in which gene expression is controlled in response to cell density. In this study an N-acylhomoserine lactone (AHL) synthase (Rru_A3396) knockout mutant (M68) of Rhodospirillum rubrum S1H (WT) was constructed and characterized phenotypically under light anaerobic conditions. Results showed that R. rubrum WT produces unsubstituted, 3-OH and 3-oxo-substituted AHLs with acyl chains ranging from 4 to 14 carbons, with 3-OH-C8 being the most abundant. Growth, pigment content and swimming motility were found to be under the control of this LuxI-type QS system. In addition, cultivation in a low shear environment put forward the aggregative phenotype of M68 and linked biofilm formation to QS in R. rubrum S1H. Interestingly, QS-mutant M68 continued to produce decreased levels of 3-OH-C8-HSL, probably due to the presence of an extra HdtS-type AHL synthase. |
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| Keywords: | Quorum sensing Pigments Biofilm Low shear environment MELiSSA HdtS |
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