艾灸对缺氧缺血性脑病新生小鼠脑组织炎症细胞浸润和 CD11b表达的影响

目的 观察艾灸对缺氧缺血性脑病新生小鼠脑组织炎症细胞浸润和CD11b表达的影响。方法 出生7 d 新生ICR小鼠，随机分为3组：假手术组（n=20）、模型组（n=24）和艾灸组（n=20）。假手术组只做颈部手术，不闭合颈 总动脉，不缺氧；模型组行颈总动脉闭合术，并给予缺氧处理；艾灸组在模型组的基础上给予艾灸治疗，每日1次，35 min/次。采用TTC染色检测小鼠脑梗死的面积；HE染色观察小鼠脑组织形态结构和炎症细胞浸润；组织免疫荧光染 色检测脑组织CD11b表达。结果 与假手术组相比，模型组小鼠的脑梗死面积增大，患侧脑组织大量细胞坏死脱 落，并伴有大量炎症细胞浸润，CD11b阳性细胞数明显增多；与模型组相比，艾灸组小鼠的脑梗死面积缩小，患侧脑组 织细胞排列较致密、整齐，炎症细胞较少，CD11b阳性细胞数明显减少。结论 艾灸具有减轻新生小鼠缺氧缺血性脑 损伤的作用，这可能与其减少脑组织小胶质细胞浸润、减轻炎症反应有关。

Effects of moxibustion on inflammatory cell infiltration and CD11b expression in brain tissue of neonatal mice with hypoxic-ischemic encephalopathy

Objective To investigate the effects of moxibustion on inflammatory cell infiltration and CD11b expression in ipsilateral brain of a neonatal mouse model with hypoxic-ischemic (HI) brain injury. Methods Postnatal 7 d ICR mice were randomly divided into three groups: sham group (n=20), HI group (n=24) and moxibustion-treated group (n=20). For sham animals, only neck surgery was performed and the common carotid artery was not occluded and was not exposed to hypoxia. For HI animals, the common carotid artery was occluded and exposed to hypoxia, while for moxibustion-treated animals, moxibustion treatment (once a day for 35 minutes) was applied in addition to HI procedure. TTC staining was used to examine area of cerebral infarction in rats, HE staining was used to detect inflammatory cells, and immunofluorescent staining was used to examine the CD11b-positive cells in the ipsilateral brain. Results Mice in HI group showed larger infarct area, more cell loss, more inflammatory cells and more CD11b-positive cells in the ipsilateral brain than those in sham group, while mice in moxibuston-treated group showed smaller infarct area, less cell loss, less inflammatory cells and less CD11b -positive cells in the ipsilateral brain than those in HI group. Conclusion Moxibustion can decrease microglia infiltration and alleviate inflammation reaction, hence reducing brain injury after HI procedure.