MenSCs移植对大鼠多囊卵巢综合征的影响及其机制

目的 探讨经血来源干细胞（MenSCs）移植对多囊卵巢综合征（PCOS）大鼠模型的治疗效果。方法 成年雌性 SD大鼠 45只均分为 3组（n=15）：对照组不作处理;PCOS组肌内注射脱氢表雄酮（DHEA,60 mg/kg）诱导 PCOS;移植组肌内注射 DHEA,饲养 3周后后经尾静脉注射 MenSCs悬液。各组饲养 5周后处死,取静脉血,酶联免疫吸附试验（ELISA）检测促卵泡激素（FSH）、黄体生成素（LH）、雌二醇（E2）、孕激素（P）、睾酮（T）、泌乳素（PRL）水平。取卵巢组织进行 HE染色观察卵巢形态。实时荧光定量检测卵巢组织 miRNA-135表达。Western blot检测卵巢组织 Bax和Bcl-2蛋白表达。结果 相对于对照组,PCOS组的 FSH降低,LH和 T均升高（P＜0.05）;相对于 PCOS组,移植组的FSH升高,LH和 T均降低（P＜0.05）。对照组卵泡形态正常,PCOS组卵巢可见较多囊状扩张的卵泡,移植组多囊状扩张的卵泡较 PCOS组减少。与对照组比较,PCOS组 miRNA-135和 Bcl-2蛋白相对表达水平降低,而 Bax蛋白相对表达水平升高（P＜0.05）;与 PCOS组比较,移植组 miRNA-135和 Bcl-2蛋白相对表达水平升高,Bax蛋白相对表达水平降低（P＜0.05）。结论 经血来源干细胞移植对大鼠 PCOS有明显的改善作用,其机制可能通过上调 miRNA-135的表达,进而调控 Bax/Bcl-2途径来实现。

The effect and mechanism of transplanted MenSCs on polycystic ovary syndrome in model rats

Objective To investigate the therapeutic effect of transplantation of menstrual stem cells (MenSCs) on ratmodel of polycystic ovary syndrome (PCOS). Methods A total of 45 female SD rats were divided into 3 groups. The control group was not treated. The PCOS group was intramuscularly injected with dehydroepiandrosterone (DHEA, 60 mg/ kg) to induce PCOS. The transplant group was intramuscularly injected with DHEA. After 3 weeks, the MenSCs suspension was injected through the tail vein. After 5 weeks of feeding, venous blood was taken. ELISA kit was used to detect folliclestimulating hormone (FSH), luteinising hormone (LH), estradiol (E2), progesterone (P), testosterone (T) and prolactinemia(PRL) levels. Ovarian tissues were taken for HE staining to observe ovarian morphology. qPCR was used to detect miRNA-135 expression in ovarian tissue. Western blot assay was used to detect the expression of Bax and Bcl-2 proteins in ovarian tissue. Results The FSH level was significantly decreased, and LH and T levels were significantly increased, in the PCOS group than those in the control group (P＜0.05). The FSH level was significantly increased, and LH and T levels were significantly decreased in the transplantation group than those in the PCOS group (P＜0.05). The follicle morphology wasnormal in the control group. Follicles with more cystic dilatation in ovary were observed in the PCOS group. The follicles with polycystic expansion were less in the transplantation group than those in the PCOS group. The expressions of miRNA-135 and Bcl-2 protein were significantly decreased in the PCOS group than those in the control group, and the expression of Bcl-2 protein was significantly higher in the PCOS group than that in the control group (P＜0.05). The expressions of miRNA-135 and Bcl-2 protein were significantly higher, the expression of Bas was significantly decreased, in the transplantation group than those of PCOS group (P＜0.05). Conclusion MenSCs has a significant improvement effect on rat PCOS, which may be achieved by up-regulating the expression of miRNA-135 and then regulating the Bax/Bcl-2 pathway.