心力衰竭大鼠解偶联蛋白2表达及其对线粒体功能的影响

目的观察心力衰竭大鼠心肌线粒体解偶联蛋白2(uncoupling protein 2,UCP2)表达及其对线粒体功能的影响。方法45只雄性SD大鼠随机分为腹主动脉缩窄组(实验组)、假手术组和正常对照组,实验组行腹主动脉缩窄术。术后20周心脏超声检测心功能,测定血清游离脂肪酸(free fatty acid,FFA)浓度,免疫印迹法分析心肌线粒体UCP2蛋白表达,密度梯度法提取大鼠心肌线粒体,氧电极法测定线粒体的呼吸活性,罗丹明123法测定线粒体膜电位。结果实验组术后20周左心室射血分数比假手术组和正常对照组降低(41.0±6.7)%vs.(84.0±2.5)%,P<0.01;(41.0±6.7)%vs.(84.0±2.3)%,P<0.01]、舒张末期内径比假手术组和正常对照组增加(6.80±0.58)mm vs.(5.20±0.78)mm,P<0.01;(6.80±0.58)mm vs.(5.00±0.67)mm,P<0.01],大鼠心力衰竭建模成功。与正常对照组和假手术组比较,实验组血清FFA浓度明显增高(372±14)μmol/L vs.(218±16)μmol l/L,P<0.01;(372±14...

Uncoupling protein 2 expression and its effects on mitochondrial function in heart failure rats

Objectives To observe uncoupling protein 2(UCP2) expression and its effects on mitochondrial function in myocardium of heart failure rats.Methods Forty five male SD rats were randomized into 3 groups: coarctation of abdominal aorta 20 week group(experiment group),sham operation group and normal control group. Myocardial function was assessed by echocardiography in 20 weeks of postligation and the plasma free fatty acid (FFA) concentrations were measured.The expression of UCP2 in mitochondria was detected by...