Low‐level resistance and clonal diversity of Pseudomonas aeruginosa among chronically colonized cystic fibrosis patients |
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| Authors: | Alex Guerra Ferreira Robson Souza Leão Ana Paula D'alincourt Carvalho‐Assef Érica Aparecida Dos Santos Ribeiro da Silva Monica de Cássia Firmida Tania Wrobel Folescu Vilma Almeida Paixão Maria Angélica Santana Fernando Antonio de Abreu E Silva Afonso Luís Barth Elizabeth Andrade Marques |
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| Affiliation: | 1. Disciplina de Microbiologia e Imunologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;2. Laboratório de Pesquisa em Infec??o Hospitalar, IOC/Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brazil;3. Hospital Universitário Pedro Ernesto, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil;4. Instituto Fernandes Figueira, Funda??o Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brazil;5. Servi?o de Pneumologia, Hospital Especializado Octávio Mangabeira, Salvador, BA, Brazil;6. Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil;7. Laboratório de Pesquisa em Resistência Bacteriana ‐ Centro de Pesquisas Experimental Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil |
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| Abstract: | A prospective study was conducted in Brazil to evaluate antimicrobial resistance patterns and molecular epidemiology of Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients with chronic lung infection. All isolates were obtained between May 2009 and June 2010 from 75 patients seen in four reference centers in Brazil: HCPA (20 patients) and HEOM (15 patients), located in southern and northeastern Brazil, respectively; IFF (20 patients) and HUPE (20 patients), both in southwestern Brazil. Antimicrobial susceptibility testing, PCR for detection of carpapenemases, and pulsed‐field gel electrophoresis (PFGE) were performed in 274 isolates. A total of 224 PFGE types were identified and no clones were found circulating among the centers or within the same center. Despite the chronic infection, most patients were colonized by intermittent clones. Only three patients (4%) maintained the same clone during the study. The resistance rates were lower than 30% for the majority of antimicrobials tested in all centers and only 17% of isolates were multiresistant. Isolates (n = 54) with reduced susceptibility to imipenem and/or meropenem presented negative results for blaSPM‐1, blaIMP?1, blaVIM, and blaKPCgenes. Our results indicate an unexpected low level of antimicrobial resistance and a high genotypic diversity among P. aeruginosa from Brazilian chronic CF patients. |
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| Keywords: | Cystic fibrosis
Pseudomonas aeruginosa
antimicrobial resistance molecular epidemiology |
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