Effect of arsenic-containing hydrocarbon on the long-term potentiation at Schaffer Collateral-CA1 synapses from infantile male rat

Arsenic-containing hydrocarbons (AsHCs) are common constituents of marine organisms and have potential toxicity to human health. This work is to study the effect of AsHCs on long-term potentiation (LTP) for the first time. A multi-electrode array (MEA) system was used to record the field excitatory postsynaptic potential (fEPSP) of CA1 before and after treatment with AsHC 360 in hippocampal slices from infantile male rats. The element content of Na, K, Ca, Mg, Mn, Cu, Zn, and As in the hippocampal slices were analyzed by elemental mass spectrometry after the neurophysiological experiment. The results showed that low AsHC 360 (1.5 μg As L⁻¹) had no effect on the LTP, moderate AsHC 360 (3.75−15 μg As L⁻¹) enhanced the LTP, and high AsHC 360 (45−150 μg As L⁻¹) inhibited the LTP. The enhancement of the LTP by promoting Ca²⁺ influx was proved by a Ca²⁺ gradient experiment. The inhibition of the LTP was likely due to damage of synaptic cell membrane integrity. This study on the neurotoxicity of AsHCs showed that high concentrations have a strong toxic effect on the LTP in hippocampus slices of the infantile male rat, which may lead to a negative effect on the development, learning, and memory.