hucMSCs transplantation promotes locomotor function recovery,reduces apoptosis and inhibits demyelination after SCI in rats |
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| Affiliation: | 1. Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran;2. Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences. Kerman, Iran;3. Neuroscience Research Center, Institute of neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran;4. Department of Biology, Faculty of Sciences, Shahid Bahonar University. Kerman, Iran;5. Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran;6. College of Medicine-Phoenix, University of Arizona, Child Health, Phoenix, USA;7. BARROW Neurological Institute at Phoenix Children’s Hospital, Phoenix Children’s Hospital, Phoenix, USA;8. Department of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Lebanon;9. Departments of Biology, Science and Research Branch, Islamic Azad University. Tehran, Iran |
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| Abstract: | AimsSpinal cord injury (SCI) can cause a variety of cells apoptosis, neurodegeneration, and eventually permanent paralysis. This study aimed to examine whether transplanting human umbilical cord mesenchymal stem cells (hucMSCs) can promote locomotor function recovery, reduce apoptosis and inhibit demyelination in SCI models.Main methodsRats were allocated into Sham group (spinal cord exposure only), SCI + PBS group (spinal cord impact plus phosphate-buffered saline (PBS) injections), SCI + hucMSCs group (spinal cord impact plus hucMSCs injections) groups. Behavioral tests, Basso-Beattie-Bresnahan locomotion scores (BBB scores), were carried out at 0, 3, 7, 14, 21, 28 days after SCI surgery. Hematoxylin-eosin staining observed spinal cord morphology. Nissl staining detected the number of nissl bodies. Myelin basic protein (MBP) and oligodendrocyte (CNPase) were examed by immunohistochemical staining. The apoptosis of oligodendrocyte and neurons were detected by immunofluorescence.ResultsThe 28-day behavioral test showed that the BBB score of rats in the SCI + hucMSCs group increased significantly, comparing to the SCI + PBS group. The numbers of nissl bodies and myelin sheath in the damaged area of SCI + hucMSCs group were also significantly increased compared to the SCI + PBS group. HucMSCs transplanting decreased the expression of protein level of Caspase-3 and Bax and increased the Bcl-2, MBP and CNPase, rescued the apoptosis of neurons and the oligodendrocyte.ConclusionThese results showed that hucMSCs can improve motor function, tissue repairing and reducing apoptosis in SCI rats. |
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