不同壳聚糖膜片兔眼前房生物相容性的实验研究

目的观察不同壳聚糖膜片在兔眼前房的相容性，评价其用作角膜内皮细胞移植载体的可行性。设计实验研究。研究对象新西兰大白兔33只。方法33只兔中30只随机分为三组，分别将脱乙酰度为63％（A组）、74％（B组）、95％（C组）的壳聚糖生物膜植入到兔的左眼前房内，右眼不做操作作为空白组；另3只兔左眼仅做前房穿刺作为对照组。使用裂隙灯显微镜观察并记录植入后3、5、7、10、14天各组兔眼前房反应。1、2,4、8周测量角膜厚度。2、4周行术眼角膜内皮镜观察。1、3个月时病理切片，苏木精一伊红染色，观察术眼的炎症反应情况。主要指标角膜厚度、内皮细胞密度、六角形细胞率、平均细胞大小。结果术后1周内，三组兔眼均出现不同程度的角膜水肿、前房渗出、虹膜充血；术后第2周，A、B组出现前房积脓、虹膜新生血管，且膜片透明度下降，至术后2个月前房积脓、虹膜新生血管基本消失。C组第2周后未出现前房积脓及虹膜新生血管，且膜透明性好。角膜测厚显示，仅术后2周时c组角膜厚度较A、B两组的角膜厚度薄（F=13．425，P=0．000），其余时间点均未见角膜厚度的差异。术后2周各膜片组术眼内皮细胞密度、六角形细胞率和细胞大小与空白组正常兔眼问均无显著性差异（P均〉0．05）；术后1个月，A、B组HE染色示膜片周围大量炎性细胞聚集，术后3个月形成炎性肉芽肿，膜片被包裹。C组术后1个月，膜片周围未发现炎症细胞的包绕，只在膜片与角膜或虹膜组织接触点上有少量的炎性细胞，3个月后膜片周围无炎症反映。结论脱乙酰度为95％的壳聚糖生物膜较脱乙酰度63％、74％者具有更好的前房生物相容性。

Biocompatibility of different chitosan membrances in rabbit anterior chamber

Objective To observe the anterior chamber compatibility of three different chitosan membranes and explore the possibility of these chitosan membranes to be the carrier of corneal endothelial cells transplantation. Design Experimental study. Participants Thirty-three New Zealand rabbits. Methods Thirty healthy rabbits were divided randomly into three groups, different degree ofdeacetylation of chitosan carrier （group A, B, C was 63%, 74%, 95%, respectively ） was implanted into the anterior chamber of the left eyes, and the right eyes were blank control group. Other three rabbits were only performed paracentesis of anterior chamber in the left eyes as controls. The reaction of ocular anterior segment was examined under the slit lamp microscope at different times in different groups. Corneal thickness was measured at postoperative 1st, 2nd, 4th and 8th week. Corneal endothelium cell density and morphology were examined under the corneal endothelia microscope at postoperative 2nd and 4th week. Corneal samples were collected for the regular histopathological examination and hematoxylin-eosin staining to observe the inflammatory reaction at postoperative 1st and 3th month. Main Outcome Measures Corneal thickness, cell density, hexagon cells rate, cell size. Results Different degrees of corneal edema, anterior chamber exudation, iris hyperemia were observed within 1 week in all three groups. Hypopyon, iris neovascularization, decreasing of membrane transparency were observed at 2nd week and nearly disappeared at 2nd month in group A and B. Hypopyon and iris neovascularization were not observed at 2nd week in group C. The corneal thickness was statistically significant thinner in group C than in group A and B only at 2nd week after operation （F=13.425,P=0.000）. No significant differences were found in cell density, hexagon ceils rate, cell size between three groups and normal rabbit eyes （all P〉0.05）. The histopathological examination showed that the inflammatory cells gathered around the membrane in 1 mont