| 补中益气汤的LC-MS分析及其对免疫抑制小鼠的调节作用 |
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| 引用本文： | 高璟春,张金超,陈瑶,柏干荣,杨梦苏,肖培根. 补中益气汤的LC-MS分析及其对免疫抑制小鼠的调节作用[J]. 医学教育探索, 2006, 0(8): 1134-1137 |
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| 作者姓名： | 高璟春 张金超 陈瑶 柏干荣 杨梦苏 肖培根 |
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| 作者单位： | 中国医学科学院中国协和医科大学药用植物研究所，香港城市大学生物及化学系，香港城市大学深圳研究院，香港城市大学生物及化学系，香港城市大学生物及化学系，中国医学科学院中国协和医科大学药用植物研究所 北京100094香港城市大学生物及化学系，香港，香港，河北大学化学与环境科学学院化学系，河北保定071002，广东深圳518057，香港，香港，北京100094 |
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| 基金项目： | 国家“863”计划基金资助项目(2003AA2Z2052) |
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| 摘 要： | 目的研究补中益气汤的主要化学成分及其对环磷酰胺免疫抑制小鼠的调节作用。方法采用LC-MS方法对补中益气汤进行了液相和质谱分析,通过MTT法测定补中益气汤对环磷酰胺免疫抑制小鼠的T、B淋巴细胞增殖和NK细胞活性的影响。结果补中益气汤中主要化学成分是多糖、黄酮类和皂苷类化合物,能够显著提高环磷酰胺免疫抑制小鼠的T、B淋巴细胞增殖能力和NK细胞活性。结论LC-MS方法可以发展成为复方有效成分研究中一个有效手段,用来阐明复方药理作用的物质基础。
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| 关 键 词： | 补中益气汤 LC-MS 免疫 环磷酰胺 |
LC-MS Analysis of Buzhongyiqi Tang and its regulating effect on immune-depressed mice |
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| GAO Jing-chun,ZHANG Jin-chao,CHEN Yao,BAI Gan-rong,YANG Meng-su,XIAO Pei-gen. LC-MS Analysis of Buzhongyiqi Tang and its regulating effect on immune-depressed mice[J]. Researches in Medical Education, 2006, 0(8): 1134-1137 |
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| Authors: | GAO Jing-chun ZHANG Jin-chao CHEN Yao BAI Gan-rong YANG Meng-su XIAO Pei-gen |
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| Affiliation: | 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100094, China; 2. Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China; 3. Department of Chemistry, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China ; 4. Shenzhen Research Institute, City University of Hong Kong, Shenzhen 518057, China |
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| Abstract: | Objective To study the main chemical constituents in Buzhongyiqi Tang (BYT) and its regulating effect on immune-depressed mice treated by cyclophosphomide (CY). Methods LC-MS Analysis of BYT, inhibitory effect of T, B lymphocyte cell proliferation and NK cell activity were observed by MTT method. Results The main chemical constituents of BYT are polysarccharides, flavonoids, and saponins, they significantly improve the T, B lymphocyte cell proliferation and NK activity in immune- depressed mice treated by CY. Conclusion The LC-MS method could be used in studying on active constituents in Chinese compound formula so as to elucidate their material bases of pharmacology. |
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| Keywords: | Buzhongyiqi Tang (BYT) LC-MS immunology cyclophosphomide (CY) |
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