芍药苷对人骨髓基质细胞HFCL蛋白质表达的作用

目的检测芍药苷对人骨髓成纤维样基质细胞系HFCL增殖及蛋白质表达的影响,探讨芍药苷补血作用的分子机制。方法采用流式细胞术和蛋白质组学方法测定芍药苷对HFCL细胞周期及蛋白质表达的影响。结果芍药苷能促进HFCL由G0/G1期进入S期,提高增殖指数,使HFCL的9种蛋白质表达上调,5种下调。上调的蛋白质有Ras相关核蛋白、核纤层蛋白A/C、异柠檬酸脱氢酶、磷酸丙糖异构酶、ATP合酶、核蛋白体蛋白质P2和CCT;下调的蛋白质有人类cc趋化因子和Bax。结论芍药苷能促进HFCL增殖,作用于HFCL多个靶点,促进细胞结构蛋白质的合成和蛋白质分子伴侣的表达,促进HFCL的能量代谢,抑制HFCL凋亡,间接发挥补血作用。

Effect of paeoniflorin on protein expression of HFCL of human bone marrow stromal cells

Objective To investigate the effects of paeoniflorin on proliferation and the protein expression of HFCL of human bone marrow stromal cells and to discuss molecular mechanism of blood enriching functions of paeoniflorin. Methods Flow cytometry and proteomics were used respectively to measure the effect of paeoniflorin on cell cycle and protein expression of HFCL. Results Paeoniflorin could promote HFCL from G_0/G_1 phase to S phase, increase proliferative index, up-regulate nine kinds of proteins, and down-regulate five kinds of proteins of HFCL. The proteins up-regulated include Ras-related nuclear protein, lamin A/C, isocitrate dehydrogenase 3 (NAD ), triosephosphate isomerase, ATP synthase, ribosomal protein P2, and chaperonin containing t-complex polypeptide 1. Conclusion Through promoting HFCL proliferation, acting on multiple protein targets, enhancing the synthesis of cytoarchitecture proteins and the expression of protein chaperonin, increasing the energy metabolism of HFCL, and suppressing the apoptosis of HFCL, paeoniflorin plays indirectly blood enriching function.