五加皮Age蛋白在正常小鼠与荷瘤小鼠体内的组织分布

目的探讨五加皮抗肿瘤成分A ge蛋白在正常小鼠与荷瘤小鼠体内的组织分布。方法用氯胺T法对A ge蛋白进行125I标记(形成125I-A ge),由尾iv小鼠后,于不同时间取组织和血液标本,测定放射cpm比值。以放射参与量(即脏器与血液中cpm比值)作为125I-A ge在组织中分布的依据。结果在一次性快速iv125I-A ge 2 h后,正常小鼠和荷瘤小鼠体内均以肾脏中125I-A ge的分布量最高,肝脏和肺部放射参与量也较高,与心脏、脾脏等其他脏器相比差异有显著性(P<0.01);尿中125I-A ge的量很高。iv同等剂量125I-A ge后,荷瘤小鼠的肾脏、肝脏和肺部组织的放射参与量比正常小鼠偏高(P<0.05)。结论五加皮A ge蛋白在小鼠体内主要分布于血流丰富的组织,主要通过泌尿系统排泄,不易透过血脑屏障。

In vivo tissue distribution of Age protein from Cortex Acanthopanacis in normal and tumor-bearing mice

Objective To determine the in vivo tissue distribution of Age protein,an antitumor component isolated from Cortex Acanthopanacis in normal and tumor-bearing mice.Methods Age protein isolated from Cortex Acanthopanacis[WTBZ] was labelled with()~(125)I(()~(125)I-Age) by chloramine-T method.The distribution of()~(125)I-Age in various organs was judged by the cpm ratio of their relative mixture of radioactivity compared to that of blood.Results Two hours after rapid iv injection of()~(125)I-Age in mice tail once,the highest concentration of()~(125)I-Age was in kidney,followed successively in liver and lung which were higher than that in heart,liver,and other organs as well,showing significant difference statistically(P<0.01).The concentration of()~(125)I-Age in the urine was rather high.In tumor-bearing mice by iv the same dose,the concentration of()~(125)I-Age in kidney,liver,and lung was higher than that in normal mice(P<0.05).Conclusion The basic characters of tissue distribution of Age protein in mice: the concentration of Age protein is higher in the tissue with abundant blood stream.The Age protein is excreted mainly by urinary system,and hard to permeate through blood-cerebral barrier.