Electrophysiologic characteristics of human and rat retinas in vitro |
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Authors: | Jun C Huang Mary J Voaden John Marshall Colin M Kemp |
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Institution: | (1) Institute of Ophthalmology, University of London, London, UK |
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Abstract: | To promote studies on the human retina, we investigated the survival of function in postmortem specimens. Visual pigment has been regenerated in normal human retinas, 5 to 58 hours postmorten, by exposure to retinal isomers in the dark. Levels from 0.1 to 0.41 nmol/ mg protein were reached. Photoresponses were obtained in 9 of 13 retinas: P III maximum amplitudes ranged from 20–398 V and thresholds, taking the criterion amplitude as 3 V, ranged from 8.8–1340 quanta/m
2. In three cases, the b-wave was also seen. The P III amplitude vs. log intensity curves gave values of n between 0.6 and 1.0, and (the stimulus intensity for a half maximal response) between 132–3700 quanta/m2. Recovery of sensitivity did not always correspond to that of maximum response. |
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Keywords: | ERG phototransduction human and rat retinas |
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