| 抑制结肠癌细胞与肝窦内皮细胞黏附的功能性抗体的初步研究 |
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| 引用本文： | 孙力超,冉宇靓,遇 珑,胡 海,钟 星,孙立新,杨治华.抑制结肠癌细胞与肝窦内皮细胞黏附的功能性抗体的初步研究[J].中国肿瘤生物治疗杂志,2008,15(4):321-325. |
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| 作者姓名： | 孙力超 冉宇靓 遇 珑 胡 海 钟 星 孙立新 杨治华 |
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| 作者单位： | 中国医学科学院,中国协和医科大学肿瘤研究所,细胞及分子生物学研究室,北京,100021 |
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的:研制抑制结肠癌细胞与肝窦血管内皮细胞黏附的功能性单抗,鉴定该单抗识别的抗原分子.方法:以人肝窦内皮细胞(human liver sinusoidal endothelial cell, HLSEC)免疫BALB/c小鼠,将小鼠脾细胞与SP2/0细胞融合,采用甲基纤维素选择培养液培养,制备抗HLSEC的单克隆抗体库.采用免疫荧光染色、黏附实验等方法筛选、鉴定能抑制结肠癌Ls174T细胞与肝窦内皮细胞黏附的功能性单克隆抗体.提取肝窦内皮细胞膜蛋白,采用Western blotting方法鉴定单抗识别的功能性抗原蛋白.结果:HLSEC免疫小鼠的脾细胞与SP2/0细胞融合后产生1 440个杂交瘤株,获得119株产生抗HLSEC抗体的阳性克隆,其中20株能显著抑制具有肝转移能力的结肠癌Ls174T细胞与HLSEC的黏附;其中15株为IgM型,3株为IgG型,2株测不到重链;其中1株抗黏附能力最强的单抗(黏附抑制率为51%)命名为12B6,该单抗在5～200 μg/ml范围内显著阻断HLSEC与Ls174T的黏附,且呈剂量依赖性;单抗12B6所识别的HLSEC抗原蛋白的相对分子质量为46 000.结论:建立了抗HLSEC单克隆抗体库,获得了20株具有抑制结肠癌细胞与肝窦血管内皮细胞黏附的功能性抗体,初步鉴定了一种可能参与结肠癌肝转移的黏附分子,为结肠癌的组织器官特异转移机制及靶向治疗的研究奠定了一定的基础.
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| 关 键 词： | 结肠癌 肝窦内皮细胞 黏附 功能性单克隆抗体 抗原 |
| 收稿时间： | 2008/5/15 0:00:00 |
| 修稿时间： | 2008/7/12 0:00:00 |
Establishment of functional antibodies for inhibition on adhesion between colon cancer and human liver sinusoidal endothelial cells |
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| SUN Li chao,RAN Yu liang,YU Long,HU Hai,ZHONG Xing,SUN Li xin and YANG Zhi Hua.Establishment of functional antibodies for inhibition on adhesion between colon cancer and human liver sinusoidal endothelial cells[J].Chinese Journal of Cancer Biotherapy,2008,15(4):321-325. |
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| Authors: | SUN Li chao RAN Yu liang YU Long HU Hai ZHONG Xing SUN Li xin and YANG Zhi Hua |
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| Institution: | Department of Cell and Molecular Biology, Cancer Institute, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100021, China;Department of Cell and Molecular Biology, Cancer Institute, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100021, China;Department of Cell and Molecular Biology, Cancer Institute, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100021, China;Department of Cell and Molecular Biology, Cancer Institute, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100021, China;Department of Cell and Molecular Biology, Cancer Institute, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100021, China;Department of Cell and Molecular Biology, Cancer Institute, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100021, China;Department of Cell and Molecular Biology, Cancer Institute, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100021, China |
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| Abstract: | |
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| Keywords: | colon cancer liver sinusoidal endothelial cell adhesion functional monoclonal antibody antigen |
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