E-钙黏附素在大鼠肝纤维化模型中的表达及意义

目的探讨大鼠实验性肝纤维化形成过程中E-钙黏附素的表达及其变化,为临床肝纤维化及肝硬化的诊断和治疗提供借鉴。方法取SD大鼠60只,其中40只按皮下注射四氯化碳每周2次,每次2 ml/kg的方法造成肝纤维化模型,另外20只皮下注射相同容量生理盐水作为正常对照组。根据造模的不同时间及肝组织的病理改变,20只造模7周后的SD大鼠设为肝纤维化组,20只造模12周后的SD大鼠设为肝硬化组。最后收集所有标本按肝纤维化0~Ⅳ期分为5组。采用免疫组织化学方法以及RT-PCR法检测肝组织中E-钙黏附素的表达及分布。结果肝纤维化0期标本仅见少量E-钙黏附素表达。与0期相比,Ⅲ期及Ⅳ期E-钙黏附素表达均增强,尤以Ⅳ期最为显著（P＆lt;0.05或P＆lt;0.01）。结论肝纤维化肝组织中E-钙黏附素的表达增强。表达量与肝纤维化程度一致。E-钙黏附素与肝纤维化的发生发展密切相关。

Expression and significance of E-cadherin in rat model of hepatic fibrosis

Objective To approach the expression of E-cadherin in rat hepatic fibrosis model, and to provide guideline for clinical diagnosis and treatment of hepatic fibrosis and cirrhosis. Methods Total 60 SD male rats were used in this experiment, among them 40 rats were administered subcutaneous injection of CCI4, 2 ml/kg, twice a week, while other 20 SD rats （as the normal control group） were given injection of normal saline. Based on the duration of CCI4 injection, 20 SD rats which received CC14 injection for 7 weeks were enrolled into hepatic fibrosis group and 20 SD rats which received subcutaneous injection of CCI4 for 12 weeks were enrolled into hepatic cirrhosis group. Finally, all specimens were collected and divided into 5 groups according to hepatic fibrosis 0 - Ⅳ stages. The expression of E-cadherin was determined by methods of immunohistochemistry and RT-PCR. Results A small quantity of E-cadherin was detected in specimens of 0 stage. Significantly more expression of E-cadherin was detected in specimens of Ⅲ and Ⅳ stages as compared with those in specimens of 0 tagse （P 〈 0.05 or P 〈 0.01 ）. Conclusion Expression of E-cadherin in rat hepatic fibrosis tissue increases. The quantity of expression of E-cadherin parallels the degree of hepatic fibrosis. Hence, E-cadherin has an intimate correlation with the occurrence and development of hepatic fibrosis.