康脑液对脑缺血再灌注损伤大鼠软脑膜微循环的影响

目的 探讨康脑液对脑缺血再灌注损伤大鼠软脑膜微循环的干预作用.方法 大鼠分为2组,干预组每日以康脑液灌胃,对照组以生理盐水代替,连续18 d后,采用颈动脉引流法复制脑缺血再灌注损伤模型,用活体显微电视录像技术,观察软脑膜微循环变化,并以田牛氏加权积分法对流态进行分析.结果 ①康脑液可改善脑缺血再灌注时软脑膜微血流的流态：造模前微血流速度快,细动脉为线流,细静脉为线粒流,无红细胞聚集,两组间未见统计学差异（P〉0.05）.造模后模型组微血流速度显著变慢（P〈0.01）,多为粒流及粒缓流,重度红细胞聚集;微动脉血流明显减少,再灌注后渗出明显.康脑液组微血流速度也变慢,但流态变化较轻（P〈0.05）,多为线粒流及粒线流,红细胞聚集及渗出也较轻,其流态积分值显著低于模型组（P〈0.05～0.01）.②康脑液可拮抗脑缺血再灌注时微血管口径的收缩：造模后模型组微血管立即收缩,细动脉以脑缺血15～30 min及再灌注后15～120 min、细静脉以脑缺血15 min收缩显著（P〈0.05～0.01）,而康脑液组微血管口径变化不明显（P〉0.05）.两组比较,康脑液组软脑膜微血管口径缩窄明显较轻（P〈0.05～0.01）.③康脑液可改善脑缺血再灌注时毛细血管的关闭：造模后虽两组毛细血管交点计数均减少（P〈0.01）,但模型组可见毛细血管大量关闭,而康脑液组毛细血管交点数显著多于模型组（P〈0.05～0.01）.结论 康脑液能减轻大鼠脑缺血再灌注时软脑膜微循环障碍,表现为对脑缺血再灌注时的微血流变慢、红细胞聚集、微血管口径缩窄及毛细血管关闭具有很好的干预作用.

Effects of Kangnaoye on Cerebral Ischemia and Reperfusion Injury in Rats

Objective To study the interference action of self-made kangnaoye(from traditional Chinese herbal medicine) on pia mater microcirculalion after cerebral ischemia and reperfusion in rats.Methods The revised method described by Bannister was adopted to establish the experimental model of rats common cartoid arteries ischemia and reperfusion injury.Twenty male Wistar rats were randomly assigned to the two groups: kangnaoye treatment group and model control group.The rats in kangnaoye group were intubated orally with 2 ml kangnaoye once daily for 18d,the kangnaoye replaced by the equivalent volume of normal saline(NS) in model group.For recording of microcirculalion images in pia mater,skull windows were performed to investigate the internal diameter of pia mater micro-vessels(A_(1),A_(2),V_(1),V_(2)),micro-blood flow condition and capillary intersections by using a vital microscope with TV recorder before and after making model .The analysis of blood flow condition by Tian's multiple score method was used.Results 1.The distubance of the pia mater micro-blood flow condition was improved.The micro-blood flow speed was very fast before making model,there was no difference between the two groups(P>0.05).The micro-blood flow speed slowed(P<0.01) and the red cell aggregated after making model,but the kangnaoye group was obviously lighter than that of model group both at ischemia and reperfusion stages(P<0.05-0.01).2.The effect of diameter contraction ofpia mater microvessels caused by cerebral ischemia and reperfusion was antagonized(P<0.05-0.01).The diameter of pia mater arterioles during ischemia 15-30 min and reperfusion 15-120 min,venules at ischemia 15 min significantly contracted in model group,but they were not changed during experiment in kangnaoye group(P>0.05).3.The capillary intersections were increased.The blood flow in arterioles decreased and many capillaries closed in model group,but the capillary intersections in kangnaoye group were more than those in model group(P<0.05-0.01).Conclusion Kangnaoye could markedly improve the pia mater microcirculalion and had a neuroprotective effect on cerebral ischemia and reperfusion injury in rats.