| 用高效液相色谱法测定“抗孕-53”中C_2-炔基异构体的含量 |
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| 引用本文： | 叶智厚,金昕. 用高效液相色谱法测定“抗孕-53”中C_2-炔基异构体的含量[J]. 生殖与避孕, 1985, 0(2) |
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| 作者姓名： | 叶智厚 金昕 |
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| 作者单位： | 上海市计划生育科学研究所(叶智厚)，上海市计划生育科学研究所(金昕) |
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| 摘 要： | “抗孕-53”是甾体口服避孕药。在合成过程中产生两个差向异构体,即“2α,17α-双炔基-A-失碳-雄烷-2β,17β-双羟基双丙酸酯”和“2β,17α-双炔基-A-失碳-雄烷-2α,17β-双羟基双丙酸酯”,用高效液相色谱法对此两异构体分离和定量测定进行了研究。用2.6mm×250mm不锈钢色谱柱,填充硅胶A,柱温30℃,以1%乙腈+1%异丙醇+98%正己烷的三元体系混合溶剂作流动相,以紫外分光光度计作检测器,可使此两异构体分离度达1.05。2α-构型化合物定量误差±3%,最低检测量2α型5×10~(-7)g,2β型2.5×10~(-7)g。分析时间15分钟。
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QUANTIFICATION OF TWO EPIMERS OF ANORDRIN BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
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| Abstract: | Anordrin is an oral steroidal contraceptive. During its process of synthesis there isa pair of epimers being produced, i. e. "2α, 17α-diethynyl-A-nor-5α-androstame 2β,17β-diol diproprionate"; "2β. 17α-diethynyl-A-nor-5α-androstame 2α, 17β-diol dipro-Prionate". The separation and quantification of the two epimers by HPLC were investigated. Aresolution factor of 1.05 was obtained by using a stainless steel column (2.6 mm×250 mm)packed with silica A and operated at 30 ℃ with a mobile phase of a ternary system of solventsand U. V. detection. The error of quantification of 2αis ±3% minimus detectable for 2αis 5×10~(-7) g and2β is 2.5×10~(-7)g. Time for analysis is 15 min. |
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