活血化瘀中药对清解通下中药增效作用的实验研究Ⅱ--对急性细菌性腹膜炎大鼠肠粘膜通透性的影响

目的:研究活血清下汤对急性细菌性腹膜炎大鼠肠粘膜通透性的影响.方法:采用盲肠结扎加穿孔法制作腹膜炎动物模型,将大鼠随机分为正常对照组、模型对照组、抗生素组、复方丹参方组、加味小承气汤组和活血清下汤组,每组6只动物,术后72 h以乳果糖(L)、甘露醇(M)混合溶液灌胃,高效液相色谱法测定二者在尿中的含量和比值,以反映肠粘膜通透性的大小.结果:细菌性腹膜炎大鼠尿中乳果糖/甘露醇比值显著高于正常组(P＜0.01),主要由于尿中乳果糖含量较高引起;各治疗组乳果糖/甘露醇比值均有所降低,尤以活血清下汤组效果显著(P＜0.05或0.01).结论:急性细菌性腹膜炎可引起肠粘膜通透性增高,活血清下汤有保护肠粘膜屏障功能的作用,使肠粘膜通透性增高受到抑制.

Experimental Study on Synergistic Action of Herbs of Huoxue Huayu(活血化瘀)Herbs on Qingjie Tongxia (清解通下) Herbs Ⅱ--Influence on Intestinal Mucosal Permeability of Acute Bacterial Peritonitis in Rats

Objective To investigate the influence of Huoxue QingxiaHXQX decoction on intestinal mucosal permeability in rats of acute bacterial peritonitis Methods Rat model of acute bacterial peritonitis was established with cecal ligation plus puncture(CLP).Animals were divided into 6 groups randomly:normal group,control group and four treatment groups (Antibiotics,Fufang DanshenFDS decoction,jiawei XiaochengqiJWXCQ decoction,and HXQX decoction).Each group contained 6 rats.Lactulose(L) and mannitol(M) were force-fed 72 hours after operation.The amounts of L and M as well as the ratio of L/M in urine were detected by high performance liquid chromatography (HPLC) to renect the permeability of intestinal mucosa. Results The ratio of L/M was higher in rats of acute bacterial peritonitis than in normal group(p<0.01).It was resulted from higher concentration of L in urine of the former.The amounts of L and M as well as the ratio of L/M in urine were decreased to different extent in treatment groups.It was more evident in the group treated with HXQX decoction(p<0.05 or 0.01). Conclusion In acute bacterial peritonitis the permeability of intestinal mucosa is changed.HXQX decoction is of potential action on protection to permeability of intestinal mucosa and enhancement of intestinal barrier.