构建7q32区域鼻咽癌和正常鼻咽上皮细胞部分基因的表达图谱

目的构建７ｑ３２区域鼻咽癌细胞和组织及原代培养人正常鼻咽上皮细胞的部分基因表达图谱。方法通过差异ＲＴ－ＰＣＲ和Ｎｏｒｔｈｅｒｎ杂交的方法检测定位于７ｑ３２区域的２０个ＥＳＴ在鼻咽癌细胞和鼻咽癌组织及原代培养人正常鼻咽上皮细胞ｍＲＮＡ的表达水平。结果８个ＥＳＴ在鼻咽癌细胞ＨＮＥ１和原代培养人正常鼻咽上皮细胞中表达量较一致，７个ＥＳＴ在两种细胞中均无表达，３个ＥＳＴ（Ｗ７２６８８、Ｈ１９８３０、ＡＡ１３０６３０）在鼻咽癌细胞株中表达上调，而２个ＥＳＴ（ＡＡ０７０４３７、Ｈ９０８８２）在原代培养人鼻咽上皮细胞中表达上调。在１３例鼻咽癌活检组织中３０．７％（４／１３）的ＡＡ０７０４３７表达下调，７７％（１０／１３）的Ｗ７２６８８和７７％（１０／１３）的Ｈ１９８３０表达上升。结论构建了７ｑ３２区域鼻咽癌细胞和组织及原代培养人正常鼻咽上皮细胞部分基因表达图谱，并初步认为Ａ０７０４３７的表达下调和Ｗ７２６８８、Ｈ１９８３０的过表达与鼻咽癌的发生有关。

ESTABLISHMENT OF PARTIAL GENE EXPRESSION MAP OF 7q32 IN NASOPHARYNGEAL CARCINOMA AND PRIMARY CULTURE NORMAL NASOPHARYNGEAL EPITHELIAL CELLS

Objective To establish partial gene expression map of 7q32 in nasopharyngeal carcinoma (NPC) cell line, tissues and primary culture normal nasopharyngeal epithelial cells.Methods We detected the expression of 20 ESTs at 7q32 in NPC cell line HNE 1, 13 NPC biopsies and primary culture normal nasopharyngeal epithelial cells using differential RT PCR and Northern hybridization.Results 8 EST (AA188181,AA13079,N27556,AA031919,N22721,H20825,T91284,AA001936)expressed equally in both of HNE 1 and primary culture normal nasopharyngeal epithelial cells; 7 EST(T64215,AA025822,R60014,R80002,H06688,R60192,R95096)expressed in neither of them; 3 EST (H19830, W72688,AA130630)overexpressed in HNE 1; and 2 EST (AA070437,H90882) overexpressed in primary culture normal nasopharyngeal epithelial cells. W72688 and H19830 each overexpressed in 77%(10/13) of NPC biopsies; AA070437 downexpressed in 30.7% of NPC biopsies.Conclusion Partial gene expression map of 7q32 in nasopharyngeal carcinoma cell line, tissues and primary culture normal nasopharyngeal epithelial cells has been established. The up regulation of W72688, H19830 and down regulation of AA070437 may be related to the occurrence of NPC.