大鼠肝郁气滞型乳腺增生病模型的建立

目的建立大鼠乳腺增生病证结合模型。方法采用激素结合束缚方法，大鼠隔2日肌肉注射苯甲酸雌二醇注射液1．0mg／kg，连续10次，后每日肌肉注射黄体酮5．0mg／kg连续5日，隔日束缚过夜共15次。末次给药次日测量大鼠乳头直径，进行旷场实验。取血液、乳腺组织备检。结果模型组大鼠乳头肿胀，垂直运动和直线运动均减少，血清PRL、E2升高，P降低，乳腺腺泡数量增多，体积增大，3周内未恢复至正常水平。结论激素结合束缚方法成功建立大鼠肝郁气滞型乳腺增生病模型，符合乳腺增生病临床发病病因，模型稳定，方法简单。

Establishment of a Cyclomastopathy Model of Stagnation of Hepatic Qi in SD Rats

Objective To establish a rat cyclomastopathy model of combination of disease and syndrome. Methods Hormone combined with restraint method is used in this study. The Estradiol Benztrone was given to rats by intramuscular injection（1.0 mg/kg） for 10 times, one time every three days. Then, the progesterone injection（5.0 mg/kg） was used for 5 days. And the rats were restrainted during the night for 15 times, performed every two days. The day after the final injection, mammilla diameter was measured, open-field behaviors were observed. Blood and mammary gland were taken for further test in all the rats. Results In model rats group, mammilla was intumescent. Both vertical and horizontal movements were reduced. High-leveled prolactin and estrodio｜ accompanied with low proges- terone were found in blood serum. Increase in number and size of lobules of mammary did not recover to the normal level in three weeks. Conclusions Hormone combined restraint method is easy to operate to establish a duplicate rat cyclomastopathy model successfully, and it is in accordance with the clinical pathogenesis.