自噬基因Beclin1表达在肾癌循环肿瘤细胞上皮-间质转化及肿瘤进展中的作用

目的探索肾癌(RCC)循环肿瘤细胞(CTCs)型别及Beclin1基因表达与RCC术后复发的相关性。方法采用CanPatrol TM CTCs检测技术,对86例可手术切除RCC患者术后2年内每3个月1次,2年后每6个月1次CTCs分型检测,对不同型别CTCs中的Beclin1基因表达进行检测,分析上述结果与患者性别、年龄、肿瘤病理分期、临床分期以及术后转移的相关性。结果81例患者检测到722个CTCs:上皮型148个,混合型381个,间质型193个,阳性率为94.2%。Beclin1的阳性率为77.8%,且Beclin1在上皮型和间质型CTCs中的表达存在显著差异(P=0.000),CTCs中Beclin1的阳性率与肿瘤是否转移有关(P<0.05),CTCs总数和间质型CTCs比例在发生转移的患者中呈上升趋势。结论RCC的CTCs在转移过程中发生上皮间质化(EMT)过程,间质型CTCs比例上升可作为肿瘤转移的预测因子;且CTCs中检测Beclin1的表达可作为肿瘤转移预测因子。

Correlation between Beclin1 expression and epithelial-mesenchymal transition in circulating tumor cells of renal carcinoma

Objective To explore the relationship between the types of circulating tumor cells(CTCs)and Beclin1 expression in renal cell carcinoma(RCC)and the recurrence of RCC.Methods The types of CTCs in 86 patients with surgically resectable RCC were identified once every 3 months within 2 years after surgery,and once every 6 months 2 years after surgery with CanPatrolTM CTCs technology.The different types of CTCs were detected,and the correlation between Beclin1 expression and patients gender,age,tumor pathological stage,clinical stage and postoperative metastasis was analyzed.Results A total of 722 CTCs were identified,including 148 epithelial,381 mixed and 193 mesenchymal types,with a positive rate of 94.2%.The positive rate of Beclin1 was 77.8%.The expression of Beclin1 was significantly different between the epithelial and mesenchymal CTCs(P=0.000).The positive rate of Beclin1 was related to tumor metastasis(P<0.05).The total number of CTCs and proportion of mesenchymal CTCs showed a rising tendency in metastatic patients.Conclusion The CTCs of RCC have epithelial-mesenchymal transition(EMT)process during metastasis,the proportion of interstitial CTCs can be used as a predictor of tumor metastasis,and the expression of Beclin1 in CTCs can also be used as a predictor of tumor metastasis.