| Effect of Celecoxib on Apoptosis of Endometrial Carcinoma Cell |
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| 作者姓名： | 生秀杰 房昭 |
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| 作者单位： | The Third Affiliated Hospital Guangzhou Medical College，Department of Gynaecology，Jiangmen Central Hospital，Guangzhou 510150，Jiangmen 529070 |
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| 摘 要： | Objective:To investigate the effect of Celecoxib on proliferation and apoptosis of the endometrial carcinoma cell HEC-1B and the effect on the expression of Fas and Survivin mRNA.Methods:The inhibition on the growth of human endometrial carcinoma cell HEC-1B was investigated by cell culture and MTT experiment when treated with different concentrations of Celecoxib.The cell apoptosis was detected by flow cytometry and DNA Ladder Electrophoresis.The change of the expression of Fas and Survivin mRNA after the treatment of Celecoxib was detected With RT-PCR.Results:Celecoxib could effectively inhibit the growth of HEC-1B cells and induce apoptosis.Survivin mRNA expression was decreased and Fas mRNA expression was increased after treating with Celecoxib.Conclusion:Celecoxib could inhibit HEC-1B cell proliferation and induce its apoptosis.
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| 关 键 词： | 肿瘤 治疗方法 子宫内膜 细胞 |
| 文章编号： | 1000-9604（2007）04-05-0244 |
| 收稿时间： | 2007-07-25 |
| 修稿时间： | 2007-09-10 |
Effect of Celecoxib on apoptosis of endometrial carcinoma cell |
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| Sheng?Xiu-jie,Fang?Zhao.Effect of Celecoxib on Apoptosis of Endometrial Carcinoma Cell[J].Chinese Journal of Cancer Research,2007,19(4):244-248. |
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| Authors: | Sheng Xiu-jie Fang Zhao |
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| Institution: | (1) The Third Affiliated Hospital, Guangzhou Medical College, Guangzhou, 510150, China;(2) Department of Gynaecology, Jiangmen Central Hospital, Jiangmen, 529070, China |
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| Abstract: | Objective:To investigate the effect of Celecoxib on proliferation and apoptosis of the endometrial carcinoma cell HEC-1B and the effect on the expression of Fas and Survivin mRNA.Methods:The inhibition on the growth of human endometrial carcinoma cell HEC-1B was investigated by cell culture and MTT experiment when treated with different concentrations of Celecoxib.The cell apoptosis was detected by flow cytometry and DNA Ladder Electrophoresis.The change of the expression of Fas and Survivin mRNA after the treatment of Celecoxib was detected With RT-PCR.Results:Celecoxib could effectively inhibit the growth of HEC-1B cells and induce apoptosis.Survivin mRNA expression was decreased and Fas mRNA expression was increased after treating with Celecoxib.Conclusion:Celecoxib could inhibit HEC-1B cell proliferation and induce its apoptosis. |
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| Keywords: | Celecoxib Endometrial carcinoma Cell proliferation Apoptosis |
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