1,2-二氯乙烷急性吸入染毒脑组织损伤形态学研究

为了研究 1 ,2 二氯乙烷 (1 ,2 -DCE)在静式吸入染毒条件下对脑组织的急性损害。采用连续静式吸入染毒大鼠 1 2h后 ,观察不同染毒剂量下受试动物脑组织的形态学变化 ;测定脑组织干湿重及含水量。结果显示 :染毒剂量为 2 .5g m3 时 ,脑组织在形态学上无明显变化 ,含水量为 80 .41 % ,与对照组 (79.82 % )相比 ,无明显差异 ;5 .0g m3 染毒组在形态学上出现轻微脑水肿 ,脑组织含水量为 82 .1 4 % ,与对照组相比有显著性差异 (P <0 .0 5) ;1 0 .0g m3 组脑组织在形态学上水肿明显 ,其含水量 (83 .65 % )与各组之间差异显著 (P <0 .0 5)。结果提示 :1 ,2 -DCE在短期大量吸入的情况下可导致动物出现脑水肿。

Study on morphology of brain tissue induced by inhale 1, 2-dichloroethane

To study acute toxicity of brain tissue caused by 1,2-dichloroethane (1,2-DCE) under static inhalation. The rats were exposed to 1,2-DCE 12 hours by consecutive static inhalation. The morphology changes of brain were observed on different dose of treated rats .Dry weight and wet weight (water content) of brain were investigated too. The results showed that it is no obvious change in morphology and water content when rats were exposed to 1,2-DCE of 2.5g/m 3. The light brain edema appeared when exposed dose was 5.0 g/m 3, and the average water content of brain (82.14%)was higher than control group (79.82%), which had significantly difference. The brain morphology of the 10.0 g/m 3 -group was significant changed compared with that of the control group. The water content of the 10.0 g/m 3 -group (83.65%)was significantly higher than that of the control group (79.82%)too. The study suggests that 1,2-DCE could result in brain edema when rats inhaled lots of 1,2-DCE in short time.