| 复方鹿仙草颗粒含量测定方法研究 |
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| 引用本文： | 耿家玲,柴文英.复方鹿仙草颗粒含量测定方法研究[J].云南中医中药杂志,2009,30(7):55-56. |
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| 作者姓名： | 耿家玲 柴文英 |
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| 作者单位： | 云南省食品药品检验所,云南,昆明,650011, |
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| 摘 要： | 目的:建立测定复方鹿仙草颗粒中苦参碱和氧化苦参碱含量的HPLC方法,用以控制复方鹿仙草颗粒的质量.方法:色谱柱为氨基柱;流动相为乙腈-无水乙醇-3%磷酸(81∶9.5∶9.5);检测波长为220nm;流速1.0mL/min.结果:苦参碱在0.0676-2.028μg范围内,氧化苦参碱0.1533-4.599μg范围内线性关系良好(r苦参碱=0.99994,r氧化苦参碱=0.99999),平均加样回收率苦参碱为102.5%,氧化苦参碱为97.4%(RSD苦参碱=2.1%,RSD氧化苦参=0.97%,n=6).结论:本法灵敏、快速、准确,可用于复方鹿仙草颗粒的质量控制.
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| 关 键 词： | 复方鹿仙草颗粒 含量测定 苦参碱 氧化苦参碱 |
Determination of the Content of Matrine and Oxymatrine in Fufang Luxiancao Granule |
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| GENG Jia-ling,CHAI Wen-ying.Determination of the Content of Matrine and Oxymatrine in Fufang Luxiancao Granule[J].Yunnan Journal of Traditional Chinese Medicine and Materia Medica,2009,30(7):55-56. |
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| Authors: | GENG Jia-ling CHAI Wen-ying |
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| Abstract: | Objective: To establish a HPLC method for the determination of the content of matrine and oxymatrine in Fufang Luxiancao Granule. Methods: The chromatographic column was NH2 column;mobile phrase was acetonitrile-ethyl alcohol-3% phosphoric acid (81:9.5:9.5) with a detection wavelength of 220nm at the flow rate of1.0 ml/min. Results: The matrine and oxymatrine showed a good liner relation at the range of 0.0676-2.028μg and at the range of 0.0676-2.028μg respectively (matrine r=0.99999) and 0.1533-4.599μg (oxymatrine r=0.99999) .The average recovery rates of the matrine and oxymatrine were 102.5% (RSD=2.1%) and 97.4% (RSD=0.97%) respectively. Conclusion: The HPLC method is sensitive, rapid and accurate and can be used to control the quality of Fufang Luxiancao Granule. |
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| Keywords: | HPLC Fufang Luxiancao Granule content determination matrine oxymatrine HPLC |
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