2型糖尿病患者血清Ghrelin水平与胰岛素抵抗的相关性研究

目的探讨2型糖尿病(T2DM)患者血清生长激素释放多肽(ghrelin)水平与胰岛素及胰岛素抵抗的关系。方法采用病例对照研究,T2DM患者(实验组)20例,健康体检者(对照组)28例。检测2组空腹血糖(FBG)、空腹胰岛素(FINS)、C肽、糖化血红蛋白(HbA1c)水平,计算胰岛素抵抗指数(HOMA-IR)=FINS(mU/L)×FBG(mmol/L)/22.5],采用酶联免疫吸附法(ELISA)检测两组血清Ghrelin水平。结果实验组FBG、FINS、HOMA-IR、HbA1c、C肽均明显高于对照组实验组四种指标分别为(9.5±2.0)mmol/L,(30±10)mU/L,10.8±6.4,7.8%±2.0%,(5.0±1.1)ng/mL,比对照组(4.1±0.2)mmol/L,(17±9)mU/L,4.7±2.0,5.5%±0.7%,(3.0±1.0)ng/mL,均P<0.01,实验组血清Ghrelin水平明显低于对照组(P<0.05),并分别与FBG、FINS、HOMA-IR、HbA1c、C肽呈负相关。结论低Ghrelin水平可作为形成胰岛素抵抗和2型糖尿病的可预见性危险因素之一。

A study of the relationship between serum Ghrelin and insulin resistance in type 2 diabetes mellitus

Objective To explore serum ghrelin concentration in patients of type 2 diabetes mellitus（T2DM）and the relationship between ghrelin and insulin resistance. Methods The investigation used a case control study. We examined the fasting blood-glucose（FBG）levels,the fasting serum insulin（FINS）,C-peptide,glycated hemoglobin（HbA1c）levels in patients of 20 T2DM and 28 control group. According to the typical HOMA model,insulin resistance index（HOMA IR）was calculated. We used ELISA to detect serum Ghrelin levels of two groups. Results The result showed that the FBG,FINS,HOMA IR,HbA1c and C-peptide levels was significantly higher in the diabetes mellitus group than that of the control group T2DM（9.5±2.0）,（30±10）mU/L,10.8±6.4,7.8%±2.0%,（5.0±1.1）ng/mL vs the control group（4.1±0.2）mmol/L,（17±9）mU/L,4.7±2.0,5.5%±0.7%（3.0±1.0）ng/mL,all P0.01. The serum Ghrelin levels of T2DM was significant lower than the control group and had negative correlation with the FBG,FINS,HOMA IR,HbA1c,C-peptide levels. Conclusion The low level of serum Ghrelin can be used as the predictability risk factor in becoming insulin resistance and T2DM.