人神经生长因子对DPN大鼠坐骨神经CGT的作用

目的　观察人神经生长因子 ( h NGF)对早期糖尿病多发性神经病 ( DPN)大鼠坐骨神经中半乳糖神经酰胺转移酶 m RNA( CGTm RNA)表达的影响。方法　用链脲佐菌素 ( STZ)一次性腹腔注射诱导糖尿病大鼠模型 ,在 DPN 4周 (造模后 4周 )腹腔注射 h NGF( 90 U .kg- 1 .d- 1 ) ,共 4周。用 RT- PCR和免疫组化方法观察坐骨神经CGTm RNA,NGFm RNA和蛋白表达。结果　 DPN 8周大鼠坐骨神经 CGTm RNA升高 ,NGFm RNA和蛋白表达减少。用 h NGF治疗的 DPN大鼠 CGTm RNA明显降低 ,NGFm RNA和蛋白表达增加。结论　 h NGF治疗能纠正早期DPN大鼠坐骨神经 CGTm RNA异常

The effect of human nerve growth factor on CGTmRNA in sciatic nervers of experimental diabetic polyneuropathy rats

Objective To observe the effect of human nerve growth factor(hNGF) on UDP-Galactose:Ceramide Galactosyltransferase mRNA (CGTmRNA) in sciatic nervers of experimental diabetic polyneuropathy rats.Methods Diabetic rat was induced by a single intraperitoneal injection of streptozotocin.After 4 weeks of diabetes,hNGF(90U/kg/d) was injected intraperitoneal for 4weeks.The level of CGTmRNA,NGFmRNA and protein expressions in sciatic nerve were measured by RT-PCR and immunohistochemistry techniques.Results After 8 weeks of diabetes,CGTmRNA expression was increased significantly, NGFmRNA and protein levels were decreased in sciatic nerves.After treatment withhNGF for 4 weeks,CGTmRNA expression was decreased,and NGFmRNA and protein levels wereincreased.Conclusion Administration of hNGF can reverse abnormal expression of CGTmRNA in sciatic nervers of experimental diabetic polyneuropathy rats.