加味圣愈汤对大鼠脑外伤后神经细胞凋亡的影响

目的:研究加味圣愈汤对脑外伤大鼠海马结构神经细胞凋亡的影响,探讨其抑制创伤性脑损伤后继发性脑损伤的作用机制。方法:采用改良的Feeney法建立脑外伤模型,将动物随机分为假手术组、脑外伤组、加味圣愈汤治疗组。72h处死后行尼氏染色,光镜下观察损伤侧海马细胞形态并计数,分别用凋亡细胞原位末端标记(TUNEL)法和免疫组织化学法观察海马神经细胞凋亡及凋亡相关基因caspase-3的蛋白表达情况。结果:加味圣愈汤治疗组与脑外伤组比较,损伤侧海马神经细胞损害减轻,细胞数明显增加,神经细胞凋亡减少,caspase-3表达减少(P<0.01)。结论:加味圣愈汤治疗可减轻脑外伤后的继发性损伤,其机制可能与抑制caspase-3的表达,减少TBI后神经细胞的凋亡有关。

Effect of modified "Shengyu" decoction on the apoptosis of neural cells after traumatic brain injury in rats

Objective: To observe the effects of modified "Shengyu" decoction(MSD) on the neuronal apoptosis in the hippocampus after traumatic brain injury(TBI) in rats,and to explore the underlying mechanisms of MSD treatment on the secondary brain injury.Methods: Rats were randomly divided into sham-operated,TBI and MSD-treated groups.TBI model was established with the modified Feeney's method,and rats were sacrificed at 72h after TBI.The morphological change of neurons in the hippocampal area were observed under the optical microscope through Nissl staining.The neuronal apoptosis and expression of protein caspase-3 in the hippocampus were determined respectively with TUNEL and immunohistochemical techniques.Result: Compared with the TBI group,treatment of MSD alleviated the damage of hippocampal neurons,increased the number of neurons,and reduced the number of TUNEL positive cells and caspase-3 positive cells(P<0.01).Conclusion: Modified "Shengyu" decoction could reduce the secondary brain injury in rats after TBI.The underlying mechanism of this effect is potentially related to inhibiting the expression of caspase-3 and thereby reducing the neuronal apoptosis.