| 人胚胎提取液生物学活性研究及临床应用展望 |
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| 引用本文： | 宋燕爽,杨毅,李川,刘洪隐. 人胚胎提取液生物学活性研究及临床应用展望[J]. 中国肿瘤临床, 1996, 23(2): 92-96 |
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| 作者姓名： | 宋燕爽 杨毅 李川 刘洪隐 |
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| 作者单位： | 天津市肿瘤研究所放射生物室 |
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| 摘 要： | 本项研究证实从４～６．５个月的胎儿组织提取的有效成份（简称ＨＦＥ）具有多种生物学活性：可促进受照射损伤的小鼠造血功能的恢复，４项检测指标包括：①骨髓有核细胞数：实验组：６５．５±１５．８，对照组：１６．４±６．２。②外周血网织红细胞百分比Ｘ±ＳＤ，实验组：１６．６±０．６０，对照组０．２±０．０４。③ＣＦＵ－ＳＸ±ＳＤ，实验组：１９．６±９．６６，对照组：１．９±２．２８。④骨髓增生程度：实验组优于对照组。对５个肿瘤细胞株和正常人二倍体细胞株２ＢＳ均有不同程度的抑制生长作用，ＥＤ５０分别为Ｈｅｌａ：６２．５μｇ／ｍｌ，ＭＣＦ－７：７．８μｇ／ｍｌ，ＨＥＰ－２：４μｇ／ｍｌ，ＳＭＭＣ７７２１：６２．５μｇ／ｍｌ，Ｔｅａ：４．５μｇ／ｍｌ，而对ＬＡ－７９５和Ａ－５４９则有轻度刺激作用。可刺激人外周血单个核细胞增殖并与ＰＨＡ，ＩＬ－２有协同作用。对氨甲喋呤引起的大鼠肝、肺损伤有一定的保护治疗作用。本项研究将为其临床应用提供实验依据。
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| 关 键 词： | HFE－人胚胎提取液，CFU－S－脾集落形成单位，MTT比色法，细胞存活率，细胞增长率 |
A Study of Biological Effects of Human Fetal Extract Both in vitro and vivo |
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| Song Yan-Shuang,Yang Yi, LiChuan et al. A Study of Biological Effects of Human Fetal Extract Both in vitro and vivo[J]. Chinese Journal of Clinical Oncology, 1996, 23(2): 92-96 |
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| Authors: | Song Yan-Shuang Yang Yi LiChuan et al |
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| Abstract: | This study confirmed that the human fetal extract(HFE) of 4-6.5months old embryo tissue had various biological activities:(1)promoted the recovery of damaged hemopoietic system in mice caused by X ray as evidenced by a)number of bone marrow nucleated cells,b)peripheral reticulocyte count,c)CFU-S,d) proliferation of bone marrow in femur;(2) inhibited growth of several tumor cells;(3)enhanced peripheral blood lymphocyte(PBL)proliferation synergistically with PHA or/and rIL-2,(4)promoted regeneration of the damaged tissue caused by methotrexate. |
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| Keywords: | Human fetal extract MTT Effect |
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