PCR直接测序法检测宫颈癌组织中人乳头瘤病毒DNA

目的 探索检测宫颈癌组织中人乳头瘤病毒(HPV)DNA的有效手段。方法 采用HPV通用引物对宫颈癌标本巾的HPVL1区基因序列进行PCR扩增，根据PCR产物序列分析对HPV分型。结果 50例宫颈癌组织HPVDNA检出率为78％，其中HPV16和HPV18型混合感染最多见。检测出1例HPV58型，其LI区基因序列与德国标准株58型比对，未发现碱基变异。结论 PCR直接测序法是对宫颈癌组织中HPV检测和分型的一种有效方法，并可检测到HPV少见的特殊类型，同时也能提供已知的HPV型别的突变信息。

Detection of human papillomavirus DNA in cervical cancer tissue by PCR and direct sequencing]

OBJECTIVE: To explore an effective method for detecting human papillomavirus (HPV) DNA in cervical cancer tissue. METHODS: HPV L1 gene fragment in cervical cancer tissue was amplified by HPV-specific PCR with consensus primers, and typing of the HPV strains was performed on the basis of sequence analysis of the PCR product. RESULTS: The positivity rates of HPV DNA was 78% in the 50 cases of cervical cancer, and mixed infection with HPV16 and HPV18 strains was the most common, which accounted for 48% on the total infections. Infection with HPV58 was detected in one case. The sequencing results showed no difference in L1 sequence between the detected samples and the standard German HPV58 strain. CONCLUSION: PCR and direct sequencing approach is effective for detecting and typing of HPV DNA in cervical cancer tissue, through which rare HPV strain or mutants of known HPV strains may not escape detection.