| 反相高效液相色谱法测定参附注射液及市售黑附片中乌头碱的含量 |
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| 引用本文： | 薛燕,李农,谢丽. 反相高效液相色谱法测定参附注射液及市售黑附片中乌头碱的含量[J]. 中国药理学通报, 2001, 17(5): 585-586 |
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| 作者姓名： | 薛燕 李农 谢丽 |
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| 作者单位： | 中国医学科学院中国协和医科大学药物研究所 |
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| 摘 要： | 目的 测定参附注射液及市售黑附片中乌头碱的含量。方法 用RP HPLC ,流动相为乙腈 醋酸铵缓冲液 (pH10 5 ) ,紫外检测波长 2 3 0nm ,流速 0 8ml·min-1。结果 对照品的线性范围 1 3 9～ 2 2 2 4 μg(r =0 9997) ;市售黑附片中的乌头碱比参附注射液中的高 3倍。结论 方法简便、快速、准确、重现性好 ,可以作为样品的检验方法
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| 关 键 词： | 乌头碱 反相高效液相色谱法 |
| 文章编号： | 1001-1978(2001)05-0585-03 |
Determination of aconitine in Shenfu injection and prepared aconite root by RP-HPLC |
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| XUE Yan,LI Nong,XIE Li. Determination of aconitine in Shenfu injection and prepared aconite root by RP-HPLC[J]. Chinese Pharmacological Bulletin, 2001, 17(5): 585-586 |
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| Authors: | XUE Yan LI Nong XIE Li |
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| Affiliation: | XUE Yan,LI Nong,XIE Li 1 |
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| Abstract: | AIM To develop an assay for the determination of aconitine in Shenfu injection and prepared aconite root. METHODS RP HPLC was applied. The mobile phase consisted of acetonitrile ammonium acetate buffer solution (50∶50,pH 10 5),the detection wavelength was 230 nm and the flow rate was 0 8 ml·min -1 . RESULTS The linearity was obtained over the range of 1 39～22 24 μg( r =0 9997),aconitine concentration in the prepared aconite root was almost 3 times higher than that in the Shenfu injection. CONCLUSION The method is simple and fast, and can be used for the quantitative analysis of aconitine. |
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| Keywords: | aconitine RP HPLC |
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