| 三七皂苷K对照品的制备研究 |
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| 引用本文： | 龚云麒,王珺,高宏涛,杨兆祥. 三七皂苷K对照品的制备研究[J]. 国外医药(植物药分册), 2014, 0(8): 869-871 |
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| 作者姓名： | 龚云麒 王珺 高宏涛 杨兆祥 |
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| 作者单位： | 昆明制药集团股份有限公司,云南昆明,650100 |
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| 基金项目： | 云南省科技厅重大科技专项(2012ZA002) |
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| 摘 要： | 目的研究三七皂苷K对照品的制备。方法利用大孔吸附树脂从三七剪口提取液中分离得三七总皂苷，再用反相柱色谱法分离得到三七皂苷K，并根据理化性质和波谱数据鉴定化合物的结构，同时运用薄层色谱（TLC）、高效液相色谱（HPLC）法检查及测定质量分数。结果三七皂苷K单体经TLC检查无杂质斑点，二维及三维HPLC测定三七皂苷K的质量分数大于99％，峰质量分数因数为998．40。结果表明上述制得的纯品为单一化合物。结论制备的三七皂苷K达到中药质量标准用化学对照品的技术要求。
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| 关 键 词： | 三七皂苷K 三七剪口 对照品 |
Preparation of notoginsenoside K reference substance |
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| GONG Yun-qi , WANG Jun , GAO Hong-tao , YANG Zhao-xiang. Preparation of notoginsenoside K reference substance[J]. , 2014, 0(8): 869-871 |
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| Authors: | GONG Yun-qi WANG Jun GAO Hong-tao YANG Zhao-xiang |
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| Affiliation: | (Kunming Pharmaceutical Group Limited Corporation, Kunming 650100, China) |
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| Abstract: | Objective To study the preparation of notoginsenoside K reference substance. Methods The total saponins in the roots and rhizomes of Panaxpseudoginseng were isolated from the roots and rhizomes of P. pseudoginseng by macroporous adsorption resin, then notoginsenoside K was isolated by reverse phase column chromatography. Their structures were identified on the basis of physicochemical properties and spectroscopic data. TLC and HPLC were employed for inspecting its purity and content. Results The TLC experiments showed the notoginsenoside K had no impurity spot. 2D and 3D HPLC showed a single peak with a purity factor of 998.4 and the content of notoginsenoside K was above 99%. Conclusion The prepared notoginsenoside K complies with the technical requirements of quality standard in Chinese materia medica with chemical reference substance. |
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| Keywords: | notoginsenoside K rhizome of Panax pseudoginseng reference substance |
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