SLP-2对前列腺癌PC-3细胞增殖和早期凋亡的影响

目的探讨SLP-2（stomatin-like protein2）基因对人前列腺癌PC-3细胞增殖和凋亡的影响。方法合成和构建SLP-2沉默和过表达载体，用Lipofectamine TM2000分别转染5组人前列腺癌PC-3细胞：pcDNA3．1-SLP-2组，空质粒pcDNA3-1组，siRNA—SLP-2组，siRNA阴性对照组以及空白对照组。采用Q-PCR和Western-Blot分别检测PC-3细胞中SLP-2的mRNA和蛋白表达量；用细胞计数试剂盒（CCK-8）检测PC-3细胞增殖；采用细胞线粒体膜电位JC-1试剂盒检测PC-3细胞的早期凋亡情况。结果转染pcDNA3．1-SLP-2质粒和siRNA-SLP-2后PC-3细胞SLP．2基因mRNA表达水平分别为（324．884±16．508）和（0．195±0．016），蛋白表达水平分别为（5．013±0．284）和（0．296±0．011），与空白对照组相比差异均有统计学意义（P〈0．05）；转染pcDNA3-1-SLP-2基因48h、72h、96h后Pc。3细胞增殖吸光度值（OD值）分别为（0．714±0．007）、（1．103±0．018）、（1．348±0．018），均显著高于空白对照组（P〈0．05）。采用siRNA沉默SLP．2基因48h、72h、96h后PC-3细胞增殖率分别为（0．571±0．004）、（0．875±0．010）、（1．044±0．008），均显著低于空白对照组（P〈0．05）；细胞凋亡结果显示，转染pcDNA3．1-SLP．2和siRNA-SLP-2的PC-3细胞早期凋亡率分别为（2．433±0．577）和（9．197±0．602），与空白对照组（4．993±0．710）相比差异均有统计学意义（P〈0．05）。结论SLP-2可促进人前列腺癌PC-3细胞的增殖，并抑制细胞的早期凋亡。

The effect of SLP-2 on the cell proliferation and early apoptosis in PC-3 cells line of prostate cancer

Objective To explore the effect of SLP-2 on cell proliferation and early apoptosis in PC-3 cells line of prostate cancer. Methods The pcDNA3.1-SLP-2 and siRNA-SLP-2 were constructed and transfected into PC-3 cells by LipofectamineTM 2000 in 5 groups： pcDNA3.1-SLP-2, pcDNA3.1, siRNA-SLP-2, mock siRNA, and blank control. Q-PCR and Western blot were used to detect the expression of SLP-2 mRNA and protein, CCK-8 kit was used to detect cell proliferation, and MMP （mitochondrial membrane potential） JC-1 kit for cell apoptosis determination. Results The mRNA expression levels of SLP-2 in pcDNA3.1-SLP-2 and siRNA-SLP-2 were （324.884±16.508） and （0.195±0.016）, respectively. The protein expression levels were （5.013±0.284） and （0.296±_0.011）, respectively. There were statistical significance in both mRNA and protein expression levels of SLP-2 between these two groups and blank control group （P〈0.05）. The OD values in pcDNA3.1-SLP-2 group were （0.714±0.007）, （1.103±0.018）, （1.348±0.018）, respectively, at 48, 72, 96 h after transfection, which were significantly higher than those of the blank control. The OD values in siRNA-SLP-2 group were （0.571±0.004）, （0.875±0.010）, （1.044±0.008）,respectively, at 48, 72, 96 h after transfection, which were significantly lower than those of the blank control. The cell apoptosis assay showed that the early apoptosis rates of pcDNA3.1-SLP-2 and siRNA-SLP-2 were （2.433±0.577） and （9.197±0.602）, respectively. There were statistical significance in the early apoptosis rates between these two groups and blank control group （P〈0.05）. Conclusion SLP-2 could promote cell proliferation and inhibit the early cell apoptosis of PC-3 cell line.