| IL-1β对ATDC5成软骨分化细胞miR-455-3p的影响 |
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| 引用本文： | 黄广鑫,张志奇,侯昌禾,黄志宇,王秉钧,余宝禧,邬培慧,傅明,杨子波,廖威明. IL-1β对ATDC5成软骨分化细胞miR-455-3p的影响[J]. 中华关节外科杂志(电子版), 2014, 0(2): 45-50 |
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| 作者姓名： | 黄广鑫 张志奇 侯昌禾 黄志宇 王秉钧 余宝禧 邬培慧 傅明 杨子波 廖威明 |
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| 作者单位： | 中山大学附属第一医院关节外科,广州510080 |
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| 基金项目： | 国家自然科学基金项目（81171759;81201388）;广东省科技计划项目（20118031300012） |
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| 摘 要： | 目的：检测IL-1β对ATDC5成软骨分化细胞miR-455-3p表达的影响，探索miR-455-3p在骨关节炎中的作用。方法诱导ATDC5细胞成软骨分化后，予10 ng/ml的IL-1β刺激，在刺激4、12、24、48 h时应用实时荧光定量PCR检测miR-455-3p、C/EBPβ和软骨特征性标记物的表达情况；并利用抑制剂IKK-NBD阻断NF-κB通路后，应用实时荧光定量PCR检测IL-1β作用下miR-455-3p的表达水平。结果在IL-1β作用下的ATDC5成软骨分化细胞中miR-455-3p、C/EBPβ和软骨退变标记物（ MMP13、ADAMTS5）均上调，而软骨基质合成标记物（ ACAN、COL2A1、SOX9）则下调，且后期更为明显；而IKK-NBD可抑制IL-1β诱导的miR-455-3p表达。结论 IL-1β可上调ATDC5成软骨分化细胞miR-455-3p的表达水平，且受NF-κB通路的调节。
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| 关 键 词： | 微RNAs 白细胞介素11β NF—κB 骨关节炎 |
IL-1 beta induces expression of miR-455-3p in differentiated ATDC5 cells partly via NF-kappa B signaling |
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| Huaag Guangxin,Zhang Zhiqi,Hou Changhe,Huang Zhiyu,Wang Bingjun,Yu Baoxi,Wu Peihui,Fu Ming,Yang Zibo,Liao Weiming. IL-1 beta induces expression of miR-455-3p in differentiated ATDC5 cells partly via NF-kappa B signaling[J]. Chinese Journal of Joint Surgery(Electronic Version), 2014, 0(2): 45-50 |
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| Authors: | Huaag Guangxin Zhang Zhiqi Hou Changhe Huang Zhiyu Wang Bingjun Yu Baoxi Wu Peihui Fu Ming Yang Zibo Liao Weiming |
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| Affiliation: | . (Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China) |
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| Abstract: | Objective To investigate the effects of IL-1 beta （ IL-1β） on the expression of miR-455-3p in the differentiated ATDC5 cells.Methods After chondrogenic differentiation , ATDC5 cells were treated with 10ng/ml recombinant murine IL-1βfor different time length.Additionally, a NF-kappaB inhibitor IKK-NBD was added to the cell culture before the IL-1βtreatment.The expression of miR-455-3p, C/EBP βand the genes related to cartilage were detected by quantitative real-time PCR.Results＆amp;nbsp;The IL-1β-treat differentiated ATDC5 cells increased the expression of miR-455-3p, C/EBP β, MMP13 and ADAMTS5, while decreased the expression of ACAN , COL2A1 and SOX9 especially in the late stage. IL-1β-induced up-regulation of miR-455-3p was inhibited by IKK-NBD.Conclusion The expression of miR-455-3p in the differentiated ATDC5 cells can be induced by IL-1βand NF-kappa B is involved in the up-regulation. |
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| Keywords: | MicroRNAs Interleukin-1 beta NF-kappaB Osteoarthritis |
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