| 抗肠道病毒71型特异性卵黄抗体的制备及鉴定 |
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| 引用本文： | 朱思思,赵肃清,李月明,温俊林. 抗肠道病毒71型特异性卵黄抗体的制备及鉴定[J]. 中国免疫学杂志, 2011, 27(10): 913-916,921. DOI: 10.3969/j.issn.1000-484X.2011.10.012 |
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| 作者姓名： | 朱思思 赵肃清 李月明 温俊林 |
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| 作者单位： | 广东工业大学轻工化工学院,广州,510006 |
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| 基金项目： | 广东省211工程计划，天河区科技计划 |
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| 摘 要： | 目的:制备特异性抗肠道病毒71型(Enterovirus 71,EV71)的卵黄抗体并检测其生物学性能,这对EV71感染手足口病的预防和治疗具有重大的意义。方法:用纯化并灭活后EV71作为抗原免疫健康产蛋鸡。采用本实验室水提综合聚乙二醇法提取纯化鸡卵黄抗体;用Bradford法测定卵黄抗体提取液的蛋白含量;还原性SDS-PAGE凝胶电泳分析测定提取蛋白的纯度及相对分子质量;分别用ELISA、双向免疫琼脂扩散检测纯化特异性卵黄抗体抗EV71效价;Western blot分析特异性卵黄抗体的免疫反应性。结果:卵黄中卵黄抗体含量达7.76 mg/ml,卵黄抗体的纯度为94.86%。初免10天后蛋黄中可检测到特异性抗EV71卵黄抗体,初免40天后ELISA检测抗体效价高达1∶20 480,双向琼脂扩散检测效价达1∶16。提取的卵黄抗体具有良好的免疫反应性,能与EV71特异性结合。结论:水提综合聚乙二醇法提取纯化得到的抗EV71卵黄抗体产量和纯度高、效价好且具有良好的免疫反应性。
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| 关 键 词： | 肠道病毒71型 卵黄抗体 手足口病 制备 鉴定 |
Preparation and identification of specific chicken egg yolk immunoglobulins against enterovirus 71 |
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| ZHU Si-Si,ZHAO Su-Qing,LI Yue-Ming,WEN Jun-Lin. Preparation and identification of specific chicken egg yolk immunoglobulins against enterovirus 71[J]. Chinese Journal of Immunology, 2011, 27(10): 913-916,921. DOI: 10.3969/j.issn.1000-484X.2011.10.012 |
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| Authors: | ZHU Si-Si ZHAO Su-Qing LI Yue-Ming WEN Jun-Lin |
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| Affiliation: | ZHU Si-Si,ZHAO Su-Qing,LI Yue-Ming,WEN Jun-Lin.Light Industry Institute of Chemical,Guangdong University of Technology,Guangzhou 510006,China |
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| Abstract: | Objective:Preparing specificity chicken egg yolk antibody(IgY) against enterovirus 71(EV71) and detecting its biological activities,this is significant for prevention and treatment of EV71 infection.Methods:Four hens were immunized three times with the purified human EV71 virus as an antigen.The immunoglobulin Y(IgY) antibody was extracted and purified from the egg yolk by water combine polyethylene glycol(water-peg) method.The yield and molecular mass of IgY were analysed by Bradford method and SDS-PAGE el... |
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| Keywords: | Enterovirus 71 IgY Hand-foot-and-mouth disease Preparation Identification |
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