| CD4~+Foxp3~+调节性T细胞在恶性血液病中的变化及机制研究 |
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| 引用本文： | 张帆,程育胜,姚红,王艳红.CD4~+Foxp3~+调节性T细胞在恶性血液病中的变化及机制研究[J].中国免疫学杂志,2011,27(12):1109-1112. |
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| 作者姓名： | 张帆 程育胜 姚红 王艳红 |
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| 作者单位： | 1. 中铁十七局中心医院,太原,030001
2. 山西医科大学,太原,030001 |
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| 摘 要： | 目的:分析CD4+Foxp3+调节性T细胞(CD4+Foxp3+Treg)在恶性血液病患者外周血的比例变化,探讨CD4+Foxp3+Treg参与恶性血液病发病的可能机制。方法:用流式细胞仪检测急性白血病、淋巴瘤患者及健康对照外周血CD4+Foxp3+Treg细胞的比例;然后用小鼠的淋巴瘤细胞EL-4和红白血病瘤细胞FBL3的培养上清液与C57BL/6小鼠脾细胞共同培养72小时,RT-PCR检测Foxp3 mRNA的表达。结果:恶性血液病患者外周血CD4+Foxp3+Treg数量显著高于正常对照(14.9±2.92)%、(5.68±1.21)%,P<0.001。小鼠EL-4和FBL3细胞上清液均能够使小鼠脾细胞Foxp3 mRNA表达水平明显增高。结论:恶性血液病患者CD4+Foxp3+Treg比例增高可能导致抗肿瘤免疫功能低下,此外肿瘤细胞分泌的可溶性物质使Foxp3表达增高,增强了CD4+Foxp3+Treg细胞的抑制功能,使肿瘤易于生长和转移。
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| 关 键 词： | 恶性血液病 CD4+Foxp3+调节性T细胞 流式细胞仪 |
Change and its mechamism of CD4+ Foxp3+ regulatory T cell in the hematological malignancies |
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| ZHANG Fan,CHENG Yu-Sheng,YAO Hong,WANG Yan-Hong.Change and its mechamism of CD4+ Foxp3+ regulatory T cell in the hematological malignancies[J].Chinese Journal of Immunology,2011,27(12):1109-1112. |
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| Authors: | ZHANG Fan CHENG Yu-Sheng YAO Hong WANG Yan-Hong |
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| Institution: | ZHANG Fan,CHENG Yu-Sheng,YAO Hong,WANG Yan-Hong.Shanxi Medical University,Taiyuan 030001,China |
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| Abstract: | Objective:To analyze the proportion of CD4+Foxp3+regulatory T cells(CD4+Foxp3+Treg) in peripheral blood of hematological malignancies patients,and to investigate the pathogenesis of CD4+Foxp3+Treg in hematological malignancies.Methods:Flow cytometer was used to analyze the frequency of CD4+Foxp3+Treg in the peripheral blood monocyte from leukemia patients,lymphoma patients and healthy control.To combine lymphoma cell EL-4 or leukemia cell FBL3 supernatant with the normal mise spleen lymphocyte.After culture... |
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| Keywords: | Hematological malignancies CD4 Foxp3 regulatory T cells Flow cytometry |
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