血管紧张素Ⅱ对人精子顶体反应的影响

目的 :探讨血管紧张素 (angiotensin ,Ang )对人精子顶体反应的影响及其可能机制。方法 :检测不同浓度 Ang 诱发的人精子顶体反应率和 Ang 引起的精子胞内钙离子的变化 ,以及血管紧张素受体 AT1拮抗剂 Losartan对其的抑制作用。结果 :Ang 在 1 0 nmol/L和 1 0 0 nmol/L时均可显著增加人精子顶体反应率 ,并引起精子胞内钙离子短暂快速升高 ,Losartan可以明显抑制这些过程。结论 :Ang 可以诱发人精子顶体反应 ,这一作用可能经 AT1受体介导 ,通过引起精子胞内钙离子升高而实现。

Effect of Angiotensin II on the Acrosome Reaction in Human Spermatozoa

Objective: To investigate the effect of angiotensin Ⅱ (Ang Ⅱ) on the acrosome reaction in human spermatozoa and to explore its mechanism. Metheds: Acrosome reaction induced by AngⅡ at different concentration was determined by triple stain. Intracellular calcium was determined according to the procedures of M.Rossato et al. (Human Reproduction, 2000,15:1 739). The inhibiting effect of Losartan, a AngⅡ blocker, was also observed.Results: AngⅡ (10 nmol/L or 100 nmol/L) significantly increased the percentage of spermatozoa undergoing the acrosome reaction. Same concentration of AngⅡ stimulated a rapid but transient increase in intracellular calcium in spermatozoa. These effects of AngⅡ were inhibited by Losartan. Conclusion: AngⅡ can stimulate the acrosome reaction in capacitated human spermatozoa. This effect may be mediated by AT1 receptor and accompanied by an increase in intracellular calcium. [WT5HZ]