| HPLC-MSn法研究次乌头碱在水中的化学反应 |
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| 引用本文： | 郗瑞云,刘永刚,张宏桂,乔延江.HPLC-MSn法研究次乌头碱在水中的化学反应[J].北京中医药大学学报,2007,30(8):539-542. |
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| 作者姓名： | 郗瑞云 刘永刚 张宏桂 乔延江 |
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| 作者单位： | 北京中医药大学中药学院,北京,100102;北京中医药大学中药学院,北京,100102;北京中医药大学中药学院,北京,100102;北京中医药大学中药学院,北京,100102 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)
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国家自然科学基金 |
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| 摘 要： | 目的研究次乌头碱在水中发生的化学反应。方法采用高效液相色谱-电喷雾-质谱/质谱(HPLC-ESI-MSn)方法,液相用梯度洗脱,质谱用正离子模式。结果在水解液中发现了4种水解产物,其准分子离子M H] 分别为574、470、498、556。结论通过对水解产物的准分子离子和碎片离子的分析,鉴定其水解产物为次乌头次碱、次乌头原碱、N-去甲基乙酰基次乌头原碱和焦次乌头次碱,并推测在水中发生脱乙酰基反应、脱乙酰基同时脱苯甲酰基反应、脱苯甲酰基同时脱N上的甲基反应、脱乙酰基同时脱水的反应。
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| 关 键 词： | 水解产物 次乌头碱 高效液相色谱-电喷雾-质谱/质谱法 |
| 修稿时间： | 2006年9月18日 |
Chemical reaction of hypaconitine in water studied by HPLC-MSn |
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| XI Rui-yun,LIU Yong-gang,ZHANG Hong-gui,et al..Chemical reaction of hypaconitine in water studied by HPLC-MSn[J].Journal of Beijing University of Traditional Chinese Medicine,2007,30(8):539-542. |
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| Authors: | XI Rui-yun LIU Yong-gang ZHANG Hong-gui |
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| Abstract: | Objective To study the chemical reaction of hypaconitine in water.Method The method of HPLC-ESI-MSn was applied in gradient elution program and the mass spectrometry was in positive ion mode.Result There were 4 hydrolysates found in the hydrolytic solution and their quasi-molecular ion were respectively at m/z 574,470,498 and 556.Conclusion Through the analysis on quasi-molecular ion and fragment ion,the hydrolysates of hypaconitine are identified as benzoylhypaconine, hypaconine,N-demethylacetylhypaconine and pyrhypaconitine.There are 4 reactions in water deduced in the procedure. |
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| Keywords: | hydrolysates hypaconitine HPLC-ESI-MSn |
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