A radioimmunoassay for the R-type binders of cobalamin. |
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Authors: | T A Morelli J A Begley C A Hall |
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Affiliation: | Hematology Research Laboratory, Veterans Administration Hospital and the Department of Medicine, Albany Medical College of Union University, Albany, N.Y. 12208 U.S.A. |
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Abstract: | A radioimmunoassay of R-type binders of cobalmin was devised and tested. The assay incorporated an antibody against purified human salivary R binder as the binding reagent. The labeled ligand was cyano[57Co]cobalamin bound to the R binder of pooled human saliva. The standard source of unlabeled ligand was also from human saliva of known R binder content. The assay was responsive to R binders of several sources, to either pure R or that of crude sources and equally to R binder saturated or unsaturated with cobalamin. It was not responsive to transcobalamin II. The assay was reproducible and reliable. |
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