化学发光微粒免疫法评价B型尿钠肽体外稳定性

目的 利用化学发光微粒免疫分析法(CMIA)检测血浆B型尿钠肽(BNP)的浓度,评价其在体外保存的稳定性.方法 用ABBOTT公司的配套BNP试剂盒,在ARCHITECT系统i2000SR上测定,观察BNP的体外稳定性.选择2011年2月四川省人民医院住院患者送检标本,采用室温和普通冰箱两种不同的保存方式,并间隔一定时间进行测定,与初次测定值进行比较,以浓度下降10%作为不稳定性判定标准,从而判断BNP的稳定性.结果 CMIA测定BNP浓度,在全血血浆中,室温下可稳定8 h,4 ℃可至少稳定12 h;在分离血浆中,室温下可稳定6 h,普通冰箱4 ℃下至少可稳定8 h.结论 BNP在常规检测中,全血血浆保存最好,室温下8 h内测定稳定性良好,如不能及时测定,则以全血血浆4 ℃保存,必须12 h内测定.

Evaluation on stability in vitro of BNP by particulate chemiluminescence immunoassay

Objective To detect the plasma concentration of B-type natriuretic peptide（BNP） by particulate chemiluminescence immunoassay（CMIA） and to evaluate the stability for preservation in vitro.Methods The BNP mating reagent kit by ABBOTT company was adopted and performed the measurement on the ARCHITECT system i2000SR.The in vitro stability of BNP was observed.The testing specimens from the inpatients in February 2011 were selected and preserved with two different methods of indoor temperature and common refrigerator（4 ℃）.The specimens were detected at different time intervals and the results were compared with the initial measured values.With the concentration fall by 10% as the judging standard of instability,the stability of BNP was evaluated.Results The BNP concentration detected by CMIA was stable for 8 h at room temperature and for at least 12 h at 4 ℃ in whole blood plasma,while for 6 h at room temperature and for at least 8 h at 4 ℃ in ordinary refrigerator in the separated plasma.Conclusion BNP in routine testing is best preserved in whole plasma,the measurement within 8 h at room temperature has better stability.If the determination is unable to be timely performed,whole plasma is best to be stored at 4 ℃ and measured within 12 h.