评价UF-500i尿沉渣分析仪在诊断尿路感染中的应用

目的 探讨UF-500i尿沉渣分析仪对尿路感染诊断的价值.方法 采集该院345例门诊、住院患者清洗外阴后取中段尿,使用UF-500i尿沉渣分析仪测定尿液白细胞和细菌数,同时进行尿液的细菌培养,以细菌培养结果为金标准,并以大于或等于10×105 CFU(集落形成单位 colony-forming units)/mL 为阳性诊断标准.利用SPSS13.0统计软件绘制受试者工作特征ROC曲线,从而计算出白细胞和细菌数在尿路感染的诊断阈cut off值,得出其灵敏度、特异性、阴/阳性预测值和假阳性/阴性率.结果 尿培养结果阳性的标本96例(27.8%),得出细菌数和白细胞数cut off值分别为130.2个/μL和29.8个/μL,其联合测定对尿路感染诊断的灵敏度,特异度,假阳性率,假阴性率分别为73.58%,95.20%,26.42%,4.80%.结论 UF-500i尿沉渣分析仪是一种简单快速、可靠的尿液筛查实验,其细菌和白细胞计数可以作为尿路感染诊断检测的良好指标.

Evaluation of Sysmex UF-500i automated urinary sediment analyzer in screening urinary tract infection

Objective To study the value of UF-500i urinary sediment analyzer on assisted diagnosis of urinary tract infection.Methods 345 specimens of midstream urine were examined for leukocyte and bacteria counts by UF-500i urinary sediment analyzer.However,bacterial culture was conducted.According to the results of bacterial culture,ROC curve was drawn with SPSS13.0 software,the cut-off value of leukocyte and bacteria counts for diagnosis of urinary tract infection were found out,and its sensitivity,specificity,positive/negative predictive value,false positive/false negative rate and accuracy were calculated.Results The positive rate of urine culture was 27.8%,and the most common pathogen was Escherichia coli.The obtained cutoff values were 130.2 bacteria/μL and 29.8 leukocytes/μL.Conclusion The UF-500i automated urinary sediment analyzer can be adopted to conduct simple,rapid and reliable urine screening tests,its bacterial and leukocyte counts may be used as the better indexes for diagnosing urinary infection.