慢性阻塞性肺病患者外周血中0D34^＋细胞数量变化

目的：观察中、重度慢性阻塞性肺病（COPD）患者外周血中CD34^＋细胞数量变化。方法：按入选标准收集25例中、重度COPD患者外周血，应用密度梯度离心法分离单个核细胞，采用CD34^＋正分选试剂盒进行磁珠纯化，用流式细胞分析仪分析其纯度，同时对入选患者行常规心电图、肺功能、心动超声及血气检查。同期16例性别、年龄无差异志愿者作为对照组，入选标准：年龄〈70岁；肺功能正常；没有临床症状；无吸烟史。结果：经过分离纯化可得到93％表达CD34^＋细胞。COPD患者组与对照组外周血中的CD34^＋细胞数分别是（19．99±11．11）×10^4、（32．75±18．25）×10^4，患者组CD34^＋细胞数明显少于对照组（P〈0．05），CD34^＋细胞数与患者病程呈负相关（r=-0．689，P〈0．05）。结论：COPD患者外周血中CD34^＋细胞数降低，与患者病程呈负相关。

Change of EPCs in perpherail blood of COPD patients

Objective. To observe the change of EPCs in perpherail blood of chronic obstructive pulmonary disease （COPD） patients. Methods. Based on the selection standard, peripheral blood was col- lected from media and severe COPD patients. Peripheral blood mononuclear cells （PBMCs） were obtained by standard Ficoll gradient centrifugation, and purified by CD34^＋ positive selection cocktail. All subjects received a complete clinical assessment including pulmonary function tests, a 12-lead ECG and blood gas analysis. The selection standard of control group was as follows, age〈70 years old, normal pulmonary function, no clinical symptoms or smoking history. Results： 93% CD34^＋ cells was obtained after isolation and purification. The count of CD34^＋ cells was （19.99±11.11） ×10^4 and （32.75±18.25）×10^4in patients group and control group, respectively. The count of patients group was significantly lower （P〈0.05）. There were negative correlation between CD34^＋ cell counts and course of COPD（r=-0. 689 ,P〈0.05）. Conclusions： The count of CD34^＋ is decreased, and is negatively correlated with course of COPD.